The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1β

Joint Authors

Laohapand, Tawee
Akarasereenont, Pravit
Palo, Titchaporn
Chotewuttakorn, Sirikul
Thaworn, Athiwat
Charoenkij, Phornnapa
Thamsermsang, Onusa
Tripatara, Pinpat

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2017-10-29

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Medicine

Abstract EN

Objective.

To investigate the modulated effects of HRF on cyclooxygenase isoform expression and its activity, using the human umbilical vein endothelial cell (HUVEC) model induced by interleukin-1 beta (IL-1β).

Methods.

Cells were treated with indomethacin (positive control), HRF, and its components at various concentrations prior to treatment with IL-1β at 24 h.

Cell viability was determined by MTT assay.

Moreover, the anti-inflammatory effects of HRF and its components through mRNA and protein expression were established using real-time quantitative PCR and Western blot, respectively.

COX activity was identified via exogenous and endogenous PGE2 productions using the EIA.

Result.

There was no cytotoxicity in HUVECs treated with HRF.

None of the experimental conditions used in the study affected the expression of COX-1, but COX-2 protein expression was inhibited at concentrations under 10 µg/mL.

Despite the significantly increased levels of exogenous PGE2, HRF had no effect on COX-2 mRNA expression.

However, the production of PGE2 was lower at a concentration of 100 µg/mL HRF than at a concentration below 10 µg/mL.

Interestingly, each component of HRF revealed different effects of the Ha-Rak formula.

Conclusion.

Our preliminary findings suggest that HRF and its components provide diverse modulation of COX-2 and PGE2 at the in vitro level.

American Psychological Association (APA)

Palo, Titchaporn& Thaworn, Athiwat& Charoenkij, Phornnapa& Thamsermsang, Onusa& Chotewuttakorn, Sirikul& Tripatara, Pinpat…[et al.]. 2017. The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1β. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1154975

Modern Language Association (MLA)

Palo, Titchaporn…[et al.]. The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1β. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1154975

American Medical Association (AMA)

Palo, Titchaporn& Thaworn, Athiwat& Charoenkij, Phornnapa& Thamsermsang, Onusa& Chotewuttakorn, Sirikul& Tripatara, Pinpat…[et al.]. The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1β. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1154975

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1154975