The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1β
Joint Authors
Laohapand, Tawee
Akarasereenont, Pravit
Palo, Titchaporn
Chotewuttakorn, Sirikul
Thaworn, Athiwat
Charoenkij, Phornnapa
Thamsermsang, Onusa
Tripatara, Pinpat
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-29
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
To investigate the modulated effects of HRF on cyclooxygenase isoform expression and its activity, using the human umbilical vein endothelial cell (HUVEC) model induced by interleukin-1 beta (IL-1β).
Methods.
Cells were treated with indomethacin (positive control), HRF, and its components at various concentrations prior to treatment with IL-1β at 24 h.
Cell viability was determined by MTT assay.
Moreover, the anti-inflammatory effects of HRF and its components through mRNA and protein expression were established using real-time quantitative PCR and Western blot, respectively.
COX activity was identified via exogenous and endogenous PGE2 productions using the EIA.
Result.
There was no cytotoxicity in HUVECs treated with HRF.
None of the experimental conditions used in the study affected the expression of COX-1, but COX-2 protein expression was inhibited at concentrations under 10 µg/mL.
Despite the significantly increased levels of exogenous PGE2, HRF had no effect on COX-2 mRNA expression.
However, the production of PGE2 was lower at a concentration of 100 µg/mL HRF than at a concentration below 10 µg/mL.
Interestingly, each component of HRF revealed different effects of the Ha-Rak formula.
Conclusion.
Our preliminary findings suggest that HRF and its components provide diverse modulation of COX-2 and PGE2 at the in vitro level.
American Psychological Association (APA)
Palo, Titchaporn& Thaworn, Athiwat& Charoenkij, Phornnapa& Thamsermsang, Onusa& Chotewuttakorn, Sirikul& Tripatara, Pinpat…[et al.]. 2017. The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1β. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1154975
Modern Language Association (MLA)
Palo, Titchaporn…[et al.]. The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1β. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1154975
American Medical Association (AMA)
Palo, Titchaporn& Thaworn, Athiwat& Charoenkij, Phornnapa& Thamsermsang, Onusa& Chotewuttakorn, Sirikul& Tripatara, Pinpat…[et al.]. The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1β. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1154975
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154975