Single Oral Acute Toxicity of Banhasasim-Tang and Its Antiobesity Effect on Diet-Induced Obese Mice and 3T3-L1 Adipocytes
Joint Authors
Seo, Chang-Seob
Yoo, Sae-Rom
Shin, Hyeun-Kyoo
Ha, Hyekyung
Jeong, Soo-Jin
Lee, Mee-Young
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We had tested antiobesity effect of 52 traditional herbal formulas in 3T3-L1 adipocyte, and Banhasasim-tang (BHSST) was chosen as one of the effective medications to inhibit triglyceride accumulation.
We investigated the antiobesity effect of BHSST on 3T3-L1 adipocytes and high-fat diet- (HFD-) induced obese mice.
In addition, we evaluated the acute toxicity of BHSST in Sprague Dawley (SD) rats.
Differentiated 3T3-L1 cells were treated with various concentrations of BHSST for 8 days.
Accumulated triglyceride level and the expressions of adipogenesis-related genes and proteins were subsequently investigated.
To evaluate the single oral toxicity of BHSST, the SD rats of each sex were administered a single dose (5000 mg/kg) of BHSST via oral gavage; the control group received vehicle only.
After a single administration, the mortality, clinical signs, gross findings, and body weight were monitored for 15 days.
Male C57BL/6J mice were fed HFD for 4 weeks to induce obesity and randomly received 50 mg/kg of Orlistat (n=12, OR), 200 mg/kg of BHSST (n=12, B200), and 1000 mg/kg of BHSST (n=12, B1000) for another 8 weeks.
BHSST suppressed the triglyceride contents and lipid accumulation in a dose-dependent manner in 3T3-L1 adipocytes.
BHSST also downregulated the adipogenesis-related gene levels and protein expression compared with those in undifferentiated adipocytes.
In a single oral dose toxicity study, there was no adverse effect on mortality, clinical signs, body weight changes, and gross findings in the treatment group.
HFD-fed mice treated with BHSST showed significantly reduced body weight gain, food efficiency ratio, and white adipose tissue weight.
The medial lethal dose (LD50) of BHSST was 5000 mg/kg/day body weight for each sex in the rats.
BHSST decreased the body weight gain in HFD-fed obese mice and inhibited triglyceride accumulation via a cascade of multiple factors at the mRNA and protein levels in 3T3-L1 adipocytes.
American Psychological Association (APA)
Yoo, Sae-Rom& Jeong, Soo-Jin& Lee, Mee-Young& Shin, Hyeun-Kyoo& Seo, Chang-Seob& Ha, Hyekyung. 2018. Single Oral Acute Toxicity of Banhasasim-Tang and Its Antiobesity Effect on Diet-Induced Obese Mice and 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1155008
Modern Language Association (MLA)
Yoo, Sae-Rom…[et al.]. Single Oral Acute Toxicity of Banhasasim-Tang and Its Antiobesity Effect on Diet-Induced Obese Mice and 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1155008
American Medical Association (AMA)
Yoo, Sae-Rom& Jeong, Soo-Jin& Lee, Mee-Young& Shin, Hyeun-Kyoo& Seo, Chang-Seob& Ha, Hyekyung. Single Oral Acute Toxicity of Banhasasim-Tang and Its Antiobesity Effect on Diet-Induced Obese Mice and 3T3-L1 Adipocytes. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1155008
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155008