Tissue Distribution Comparison of Six Active Ingredients from an Eucommiae Cortex Extract between Normal and Spontaneously Hypertensive Rats
Joint Authors
Gong, Zipeng
Li, YueTing
Huang, Yong
Zheng, Lin
Wang, Ai-Min
Li, Yong-Jun
Chen, Si-Ying
Hu, Hejia
Xiao, Hongqin
Bao, Hongsong
Li, Mei
Xue, Cun
Wang, Guangcheng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-06-08
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Eucommiae Cortex (EC), a rare, nourishing medicinal herb that is native in China, has good effect in the treatment of hypertension.
In this study, we compared tissue distribution of six representative active components of EC extract—genipinic acid (GA), protocatechuic acid (PCA), neochlorogenic acid (NCA), chlorogenic acid (CA), (+)-pinoresinol di-O-β-D-glucopyranoside (PDG), and (+)-pinoresinol 4′-O-β-D-glucopyranoside (PG)—between normal rats and spontaneously hypertensive rats (SHRs).
Each rat was intragastrically given EC extract at a dose of 5.4 g/kg.
Rats were sacrificed at 10 min, 30 min, 2 h, and 8 h after administration; six rats were sacrificed at each time point.
Then, we quickly harvested their major organs, including heart, liver, spleen, lungs, kidneys, and brain.
Using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), we determined the levels of the above mentioned six components in the organs of both types of rats and then analyzed differences in the tissue distribution.
The results showed that levels of each component differed between SHRs and the normal group at each time point.
As time progressed, the number of organs in which GA distribution in each tissue of SHRs differed from that of the normal group gradually increased: SHRs showed lower GA levels than normal rats.
Levels of PG and PDG in both groups at 10 and 30 min were similar.
NCA and CA in the SHR group and the normal group at 10 min, 30 min, and 2 h were also similar to some extent.
The results indicated that the pathological state of spontaneous hypertension could affect tissue distribution of EC active components in rats.
American Psychological Association (APA)
Hu, Hejia& Xiao, Hongqin& Bao, Hongsong& Li, Mei& Xue, Cun& Li, YueTing…[et al.]. 2020. Tissue Distribution Comparison of Six Active Ingredients from an Eucommiae Cortex Extract between Normal and Spontaneously Hypertensive Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1155062
Modern Language Association (MLA)
Hu, Hejia…[et al.]. Tissue Distribution Comparison of Six Active Ingredients from an Eucommiae Cortex Extract between Normal and Spontaneously Hypertensive Rats. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-16.
https://search.emarefa.net/detail/BIM-1155062
American Medical Association (AMA)
Hu, Hejia& Xiao, Hongqin& Bao, Hongsong& Li, Mei& Xue, Cun& Li, YueTing…[et al.]. Tissue Distribution Comparison of Six Active Ingredients from an Eucommiae Cortex Extract between Normal and Spontaneously Hypertensive Rats. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-16.
https://search.emarefa.net/detail/BIM-1155062
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155062