Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats
Joint Authors
Yi, Ruokun
Zhao, Xin
Liu, Xinhong
Zheng, Jiazhuang
Li, Fang
Mu, Jianfei
Tan, Fang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A rat model of secondary osteoporosis was constructed using retinoic acid as an inducer, and the genes, proteins, and bone mass of the rats were analyzed.
qPCR detection of the Wnt/β-catenin and OPG/RANK/RANKL signaling pathway-related gene expression levels showed that Lactobacillus plantarum HFY15 played a positive role in regulating both pathways.
HFY15 significantly increased β-catenin, Lrp5, Lrp6, Wnt10b, OPG, RANKL, and Runx2 expression and downregulated DKK1, RANK, CTSK, TRACP, and ALP expression.
Enzyme-linked immunosorbent assays further confirmed the qPCR results.
Tartrate-resistant acid phosphatase staining showed that HFY15 slowed retinoic acid-induced osteoclast formation.
Microcomputed tomography showed that HFY15 reduced trabecular separation and increased the percent bone volume, trabecular numbers, trabecular thickness, and bone mineral density in the rats in vivo.
These findings indicate that HFY15 may help prevent retinoic acid-induced secondary osteoporosis in vivo.
American Psychological Association (APA)
Liu, Xinhong& Zheng, Jiazhuang& Li, Fang& Yi, Ruokun& Mu, Jianfei& Tan, Fang…[et al.]. 2020. Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1155066
Modern Language Association (MLA)
Liu, Xinhong…[et al.]. Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1155066
American Medical Association (AMA)
Liu, Xinhong& Zheng, Jiazhuang& Li, Fang& Yi, Ruokun& Mu, Jianfei& Tan, Fang…[et al.]. Lactobacillus Plantarum HFY15 Helps Prevent Retinoic Acid-Induced Secondary Osteoporosis in Wistar Rats. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1155066
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155066