Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels
Joint Authors
Wang, Minggang
Mao, Dewen
Shi, Qinglan
Zhang, Rongzhen
Qiu, Hua
Long, Fuli
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aim of the Study.
To investigate the preventative effects of Jiedu Huayu (JDHY) on D-galactosamine (D-GalN) and lipopolysaccharide-induced acute liver failure (ALF) and to evaluate the possible mechanisms of action.
Materials and Methods.
ALF was induced in Wistar rats by administrating D-GalN (900 mg/kg) and lipopolysaccharide (10 μg/kg).
After treatment with JDHY granules, the levels of blood alanine aminotransferase, aspartate aminotransferase, total bilirubin, and prothrombin time were determined.
Proliferating cell nuclear antigen was detected by immunohistochemistry staining.
The expression of interleukin-2 (IL-2) and toll-like receptor 4 (TLR4) was examined by fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot.
Results.
JDHY treatment dramatically improved liver function and increased survival rates in an ALF model in rats.
We observed a decrease in IL-2 and TLR4 expression following treatment with JDHY in liver cells from ALF rats using qRT-PCR and Western blot analysis.
Conclusion.
We hypothesize that the therapeutic potential of JDHY for treating ALF is due to its modulatory effect on the suppression of inflammation and by promoting hepatocyte regeneration.
Our results contribute towards validation of the traditional use of JDHY in the treatment of liver disease.
American Psychological Association (APA)
Wang, Minggang& Shi, Qinglan& Zhang, Rongzhen& Qiu, Hua& Mao, Dewen& Long, Fuli. 2017. Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1155087
Modern Language Association (MLA)
Wang, Minggang…[et al.]. Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1155087
American Medical Association (AMA)
Wang, Minggang& Shi, Qinglan& Zhang, Rongzhen& Qiu, Hua& Mao, Dewen& Long, Fuli. Herbal Compound “Jiedu Huayu” Reduces Liver Injury in Rats via Regulation of IL-2, TLR4, and PCNA Expression Levels. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1155087
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155087