![](/images/graphics-bg.png)
Herb-Drug Interaction: Application of a UPLC-MSMS Method to Determine the Effect of Polygonum capitatum Extract on the Tissue Distribution and Excretion of Levofloxacin in Rats
Joint Authors
Gong, Zipeng
Pan, Jie
Li, YueTing
Zheng, Lin
Wang, Ai-Min
Yuan, Li
Chen, Hao
Ma, Xue
Chen, Si-Ying
Huang, Yong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Polygonum capitatum has unique curative effects on the urinary system.
In fact, many Polygonum capitatum-based preparations are currently used in the clinic.
In China, the combination of levofloxacin (LVFX) with a Chinese herbal preparation derived from Polygonum capitatum has been used for the clinical treatment of urinary system diseases, which can improve the curative effects and reduce the side effects of LVFX.
However, the herb-drug interaction (HDI) between these drugs has not been reported and the effect of Polygonum capitatum on the in vivo process of LVFX is unclear.
In this article, a sensitive ultraperformance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) method was developed to evaluate the effects of the combined application of LVFX and the Polygonum capitatum extract on tissue distribution and excretion.
Thereafter, the method was validated for selectivity, accuracy, precision, linearity, lower limit of quantification (LLOQ), dilution integrity, recovery, and matrix effect.
Based on tissue distribution, LVFX could diffuse into all of the tested tissues, with significant differences in the content of each tissue between the coadministration group and single administration group.
At 48 h after the combination was orally administered, the urinary cumulative excretion of LVFX decreased from 20.69% to 11.84% while its fecal cumulative excretion decreased from 26.08% to 13.28%.
Our results suggest that a drug interaction exists between the two drugs in the process of distribution and excretion.
This study provides important experimental evidence for further studies on the clinical efficacy and mechanism of the Polygonum capitatum extract and LVFX.
American Psychological Association (APA)
Yuan, Li& Chen, Hao& Ma, Xue& Pan, Jie& Gong, Zipeng& Chen, Si-Ying…[et al.]. 2020. Herb-Drug Interaction: Application of a UPLC-MSMS Method to Determine the Effect of Polygonum capitatum Extract on the Tissue Distribution and Excretion of Levofloxacin in Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1155110
Modern Language Association (MLA)
Yuan, Li…[et al.]. Herb-Drug Interaction: Application of a UPLC-MSMS Method to Determine the Effect of Polygonum capitatum Extract on the Tissue Distribution and Excretion of Levofloxacin in Rats. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1155110
American Medical Association (AMA)
Yuan, Li& Chen, Hao& Ma, Xue& Pan, Jie& Gong, Zipeng& Chen, Si-Ying…[et al.]. Herb-Drug Interaction: Application of a UPLC-MSMS Method to Determine the Effect of Polygonum capitatum Extract on the Tissue Distribution and Excretion of Levofloxacin in Rats. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1155110
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155110