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Simultaneous Quantification of Gallic Acid, Bergenin, Epicatechin, Epicatechin Gallate, Isoquercitrin, and Quercetin-3-Rhamnoside in Rat Plasma by LC-MSMS Method and Its Application to Pharmacokinetics after Oral Administration of Ardisia japonica Extract
Joint Authors
Chang, Yanxu
Li, Jin
Yu, Xie-an
Teye Azietaku, John
Hao, Jia
Wang, Hui
Zheng, Fang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Ardisia japonica is a well-known traditional Chinese medicinal herb used as a diuretic, for treating cough and for stopping uterine bleeding.
A simple, sensitive, and reliable LC-MS/MS method was developed to determine six active compounds in rat plasma and this method was further applied to the pharmacokinetic study of these compounds after oral administration of Ardisia japonica extract.
Acetonitrile was used to precipitate the protein in the plasma samples.
Using acetonitrile and formic acid aqueous solution (0.05%) as the mobile phase, the separation of the six compounds and internal standards was achieved at a flow rate of 300 μL min−1 on an Eclipse plus C18 column at an elution time of 16 min.
A tandem mass spectrometer having an electrospray ionization (ESI) source was used in the detection of the analytes and internal standards using multiple reactions monitoring (MRM) in the negative ionization mode.
The LLOQ was 2, 2, 4, 2, 1, and 0.4 ng mL−1 for gallic acid, bergenin, epicatechin, epicatechin gallate, isoquercitrin, and quercetin-3-rhamnoside, respectively.
The validated method was applied to the pharmacokinetic study of gallic acid, bergenin, and quercetin-3-rhamnoside in rat plasma after oral administration of A.
japonica extract to rats.
American Psychological Association (APA)
Yu, Xie-an& Teye Azietaku, John& Li, Jin& Wang, Hui& Zheng, Fang& Hao, Jia…[et al.]. 2018. Simultaneous Quantification of Gallic Acid, Bergenin, Epicatechin, Epicatechin Gallate, Isoquercitrin, and Quercetin-3-Rhamnoside in Rat Plasma by LC-MSMS Method and Its Application to Pharmacokinetics after Oral Administration of Ardisia japonica Extract. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1155363
Modern Language Association (MLA)
Yu, Xie-an…[et al.]. Simultaneous Quantification of Gallic Acid, Bergenin, Epicatechin, Epicatechin Gallate, Isoquercitrin, and Quercetin-3-Rhamnoside in Rat Plasma by LC-MSMS Method and Its Application to Pharmacokinetics after Oral Administration of Ardisia japonica Extract. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1155363
American Medical Association (AMA)
Yu, Xie-an& Teye Azietaku, John& Li, Jin& Wang, Hui& Zheng, Fang& Hao, Jia…[et al.]. Simultaneous Quantification of Gallic Acid, Bergenin, Epicatechin, Epicatechin Gallate, Isoquercitrin, and Quercetin-3-Rhamnoside in Rat Plasma by LC-MSMS Method and Its Application to Pharmacokinetics after Oral Administration of Ardisia japonica Extract. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1155363
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155363