Ginsenoside Rg1 Alleviates Podocyte Injury Induced by Hyperlipidemia via Targeting the mTORNF-κBNLRP3 Axis
Joint Authors
Wang, Xiaolei
Xu, Jiayi
Shi, Yimin
Wu, Bingjie
Li, Yimeng
Wang, Tao
Gao, Yanbin
Yue, Rongchuan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-09
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Background.
Podocyte injury plays an important role in diabetic nephropathy (DN).
The aim of this study was to determine the potential therapeutic effects of the ginsenoside Rg1 on hyperlipidemia-stressed podocytes and elucidate the underlying mechanisms.
Methods.
In vitro and in vivo models of DN were established as previously described, and the expression levels of relevant markers were analyzed by Western blotting, real-time Polymerase Chain Reaction (PCR), immunofluorescence, and immunohistochemistry.
Results.
Ginsenoside Rg1 alleviated pyroptosis in podocytes cultured under hyperlipidemic conditions, as well as in the renal tissues of diabetic rats, and downregulated the mammalian target of rapamycin (mTOR)/NF-κB pathway.
In addition, Rg1 also inhibited hyperlipidemia-induced NLRP3 inflammasome in the podocytes, which was abrogated by the mTOR activator L-leucine (LEU).
The antipyroptotic effects of Rg1 manifested as improved renal function in the DN rats.
Conclusion.
Ginsenoside Rg1 protects podocytes from hyperlipidemia-induced damage by inhibiting pyroptosis through the mTOR/NF-κB/NLRP3 axis, indicating a potential therapeutic function in DN.
American Psychological Association (APA)
Wang, Tao& Gao, Yanbin& Yue, Rongchuan& Wang, Xiaolei& Shi, Yimin& Xu, Jiayi…[et al.]. 2020. Ginsenoside Rg1 Alleviates Podocyte Injury Induced by Hyperlipidemia via Targeting the mTORNF-κBNLRP3 Axis. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1155381
Modern Language Association (MLA)
Wang, Tao…[et al.]. Ginsenoside Rg1 Alleviates Podocyte Injury Induced by Hyperlipidemia via Targeting the mTORNF-κBNLRP3 Axis. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1155381
American Medical Association (AMA)
Wang, Tao& Gao, Yanbin& Yue, Rongchuan& Wang, Xiaolei& Shi, Yimin& Xu, Jiayi…[et al.]. Ginsenoside Rg1 Alleviates Podocyte Injury Induced by Hyperlipidemia via Targeting the mTORNF-κBNLRP3 Axis. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1155381
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155381