Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis
Joint Authors
Li, Yu
Ding, Nana
Ding, Xuedong
Wang, Xichun
Wu, Jinjie
Feng, Shibin
He, Yanting
Li, Cheng
Li, Manman
Ding, Hongyan
Li, Jinchun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Berberine hydrochloride is an isoquinoline type alkaloid extracted from Berberidaceae, Rutaceae, and other plants.
Previous reports have shown that berberine hydrochloride has anti-inflammatory properties.
However, the underlying molecular mechanisms remain unclear.
In this study, a lipopolysaccharide- (LPS-) induced murine model of mastitis was established to explore the anti-inflammatory action of berberine hydrochloride.
Sixty mice that had been lactating for 5–7 days were randomly divided into six groups, including control, LPS, three berberine hydrochloride treatment groups (5, 10, and 20 mg/kg), and a dexamethasone (DEX) (5 mg/kg) group.
Berberine hydrochloride was administered intraperitoneally 1 h before and 12 h after LPS-induced mastitis, and all mice were sacrificed 24 h after LPS induction.
The pathological and histopathological changes of the mammary glands were observed.
The concentrations and mRNA expressions of TNF-α, IL-1β, and IL-6 were measured by ELISA and qRT-PCR.
The activation of TLR4 and NF-κB signaling pathways was analyzed by Western blot.
Results indicated that berberine hydrochloride significantly attenuated neutrophil infiltration and dose-dependently decreased the secretion and mRNA expressions of TNF-α, IL-1β, and IL-6 within a certain range.
Furthermore, berberine hydrochloride suppressed LPS-induced TLR4 and NF-κB p65 activation and the phosphorylation of I-κB.
Berberine hydrochloride can provide mice robust protection from LPS-induced mastitis, potentially via the TLR4 and NF-κB pathway.
American Psychological Association (APA)
Wang, Xichun& Feng, Shibin& Ding, Nana& He, Yanting& Li, Cheng& Li, Manman…[et al.]. 2018. Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1155432
Modern Language Association (MLA)
Wang, Xichun…[et al.]. Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1155432
American Medical Association (AMA)
Wang, Xichun& Feng, Shibin& Ding, Nana& He, Yanting& Li, Cheng& Li, Manman…[et al.]. Anti-Inflammatory Effects of Berberine Hydrochloride in an LPS-Induced Murine Model of Mastitis. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1155432
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155432