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Activation of the Nrf2HO-1 Pathway by Amomum villosum Extract Suppresses LPS-Induced Oxidative Stress In Vitro and Ex Vivo
Joint Authors
Park, Sun-Dong
Kim, Jaieun
Lim, Dong-Woo
Kim, Hyuck
Park, Won-Hwan
Choi, Hee-Jin
Yu, Ga-Ram
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-04
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Despite its deleterious effects on living cells, oxidative stress plays essential roles in normal physiological processes and provides signaling molecules for cell growth, differentiation, and inflammation.
Macrophages are equipped with antioxidant mechanisms to cope with intracellular ROS produced during immune response, and Nrf2 (NF-E2-related factor 2)/HO-1 (heme oxygenase-1) pathway is an attractive target due to its protective effect against ROS-induced cell damage in inflamed macrophages.
We investigated the effects of ethanol extract of A.
villosum (AVEE) on lipopolysaccharide- (LPS-) stimulated inflammatory responses generated via the Nrf2/HO-1 signaling pathway in murine peritoneal macrophages and RAW 264.7 cells.
AVEE was found to suppress the NF-κB signaling pathway, thus, to reduce proinflammatory cytokine, nitric oxide, and prostaglandin levels in peritoneal macrophages and Raw 264.7 cells treated with LPS, and to enhance HO-1 expression by activating Nrf2 signaling.
Furthermore, these anti-inflammatory effects of AVEE were diminished when cells were pretreated with SnPP (a HO-1 inhibitor).
HPLC analysis revealed AVEE contained quercetin, a possible activator of the Nrf2/HO-1 pathway.
These results show A.
villosum ethanol extract exerts anti-inflammatory effects by activating the Nrf2/HO-1 pathway in LPS-stimulated macrophages.
American Psychological Association (APA)
Lim, Dong-Woo& Choi, Hee-Jin& Park, Sun-Dong& Kim, Hyuck& Yu, Ga-Ram& Kim, Jaieun…[et al.]. 2020. Activation of the Nrf2HO-1 Pathway by Amomum villosum Extract Suppresses LPS-Induced Oxidative Stress In Vitro and Ex Vivo. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155445
Modern Language Association (MLA)
Lim, Dong-Woo…[et al.]. Activation of the Nrf2HO-1 Pathway by Amomum villosum Extract Suppresses LPS-Induced Oxidative Stress In Vitro and Ex Vivo. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1155445
American Medical Association (AMA)
Lim, Dong-Woo& Choi, Hee-Jin& Park, Sun-Dong& Kim, Hyuck& Yu, Ga-Ram& Kim, Jaieun…[et al.]. Activation of the Nrf2HO-1 Pathway by Amomum villosum Extract Suppresses LPS-Induced Oxidative Stress In Vitro and Ex Vivo. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155445
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155445