Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21TIMP3 Axis
Joint Authors
Li, Jingtao
Wei, Hailiang
Liu, Yonggang
Li, Qian
Guo, Hui
Guo, Yingjun
Chang, Zhanjie
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background/Aim.
Curcumin exhibits anticancer effects against various types of cancer including hepatocellular carcinoma (HCC).
miR-21 has been reported to be involved in the malignant biological properties of HCC.
However, whether miR-21 plays a role in curcumin-mediated treatment of HCC is unknown.
The purpose of this study was to identify the potential functions and mechanisms of miR-21 in curcumin-mediated treatment of HCC.
Methods.
The anticancer effects of curcumin were assessed in vivo and in vitro.
The underlying mechanism of miR-21 in curcumin-mediated treatment of HCC was assessed by quantitative real-time PCR (RT-qPCR), western blot, and Dual-Luciferase Reporter assays.
Results.
The present study revealed that curcumin suppressed HCC growth in vivo and inhibited HCC cell proliferation and induced cell apoptosis in a dose-dependent manner in vitro.
Meanwhile, the curcumin treatment can downregulate miR-21 expression, upregulate TIMP3 expression, and inhibit the TGF-β1/smad3 signaling pathway.
miR-21 inhibition enhanced the effect of curcumin on cell proliferation inhibition, apoptosis, and TGF-β1/smad3 signaling pathway inhibition in HepG2 and HCCLM3 cells.
It demonstrated that TIMP3 was a direct target gene of miR-21.
Interestingly, the effect of miR-21 inhibition on cell proliferation, apoptosis, and TGF-β1/smad3 signaling pathway in HepG2 and HCCLM3 cells exposed to curcumin was attenuated by TIMP3 silencing.
Conclusion.
Taken together, the present study suggests that miR-21 is involved in the anticancer activities of curcumin through targeting TIMP3, and the mechanism possibly refers to the inhibition of TGF-β1/smad3 signaling pathway.
American Psychological Association (APA)
Li, Jingtao& Wei, Hailiang& Liu, Yonggang& Li, Qian& Guo, Hui& Guo, Yingjun…[et al.]. 2020. Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21TIMP3 Axis. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155461
Modern Language Association (MLA)
Li, Jingtao…[et al.]. Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21TIMP3 Axis. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1155461
American Medical Association (AMA)
Li, Jingtao& Wei, Hailiang& Liu, Yonggang& Li, Qian& Guo, Hui& Guo, Yingjun…[et al.]. Curcumin Inhibits Hepatocellular Carcinoma via Regulating miR-21TIMP3 Axis. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155461
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155461