Establishment of Fluorescence Sensitization Method for Hydroxysafflor Yellow A
Joint Authors
Qin, Xiu-de
Haiyan, CAO
Chen, LIU
Xinzhe, ZHAO
Yuhui, MA
Jingna, ZHOU
Yu, LIU
Shaoqin, GE
Guowei, ZHANG
Hailiang, NIE
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
As the main active ingredient in Chinese medicine safflower, hydroxysafflor yellow A (HSYA) has multiple pharmacological effects.
In the work, the absorption and fluorescence spectra of HSYA under different environmental conditions (such as acidity, temperature, ions, viscosity, and surfactant) were investigated.
The fluorescence intensity of HSYA varied greatly with acidity, temperature, viscosity, and surfactant, but was less affected by common cations and anions.
Among various surfactants, we found that borax can significantly enhance the HSYA fluorescence intensity, and thus, a borax-HSYA sensitization system for HSYA fluorescence was established.
In the optimized sensitization system, the fluorescence intensity of HSYA increased by 20 times and showed a good linearity with HSYA concentrations in the range of 0∼10 μM with a detection limit of 8 nM.
The borax-HSYA sensitization system is nontoxic to T24 cells and mice and can be used for the fluorescence imaging of HSYA in cells, thereby providing an effective method for analyzing HSYA in vitro and monitoring its metabolism in cells.
American Psychological Association (APA)
Haiyan, CAO& Qin, Xiu-de& Chen, LIU& Xinzhe, ZHAO& Yuhui, MA& Jingna, ZHOU…[et al.]. 2020. Establishment of Fluorescence Sensitization Method for Hydroxysafflor Yellow A. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155526
Modern Language Association (MLA)
Haiyan, CAO…[et al.]. Establishment of Fluorescence Sensitization Method for Hydroxysafflor Yellow A. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1155526
American Medical Association (AMA)
Haiyan, CAO& Qin, Xiu-de& Chen, LIU& Xinzhe, ZHAO& Yuhui, MA& Jingna, ZHOU…[et al.]. Establishment of Fluorescence Sensitization Method for Hydroxysafflor Yellow A. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1155526
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155526