Danggui Sini Decoction Protected Islet Endothelial Cell Survival from Hypoxic Damage via PI3KAkteNOS Pathway
Joint Authors
Li, Xuejun
Yang, Shuyu
Chen, Wenting
Huang, Caoxin
Yang, Chen
Ge, Xilin
Huang, Wenfang
Liu, Suhuan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Danggui Sini decoction (DSD) is a traditional Chinese decoction, which is wildly applied and showed to be effective in ameliorating ischemia-related symptoms.
However, the mechanisms of DSD action in ischemic damage remain to be fully clarified.
Pancreatic islet endothelial cells are pivotal constituent of islet microvasculature, with high vulnerability to hypoxic injuries.
Here, using MST1 cell, a pancreatic islet endothelial cell-line, as a model, we investigated the effects of DSD on hypoxia-stimulated endothelial cell lesions and its underlying mechanisms.
We found that DSD-Containing Serum (DSD-CS), collected from DSD-treated rats, could efficiently protect MST1 survival and proliferation from Cobalt chloride (CoCl2) induced damage, including cell viability, proliferation, and tube formation.
Furthermore, DSD-CS restored the activity of PI3K/Akt/eNOS signaling inhibited by CoCl2 in MST1 cells.
The protective effect of DSD-CS could be blocked by the specific PI3K/Akt/eNOS inhibitor LY294002, suggesting that DSD-CS protection of MST1 cell survival from hypoxia was mediated by PI3K/Akt/eNOS pathway.
In conclusion, DSD treatment protected MST1 survival from hypoxic injuries via PI3K/Akt/eNOS pathway, indicating its role in protecting microvascular endothelial cells.
American Psychological Association (APA)
Chen, Wenting& Huang, Caoxin& Yang, Chen& Ge, Xilin& Huang, Wenfang& Li, Xuejun…[et al.]. 2018. Danggui Sini Decoction Protected Islet Endothelial Cell Survival from Hypoxic Damage via PI3KAkteNOS Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155529
Modern Language Association (MLA)
Chen, Wenting…[et al.]. Danggui Sini Decoction Protected Islet Endothelial Cell Survival from Hypoxic Damage via PI3KAkteNOS Pathway. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1155529
American Medical Association (AMA)
Chen, Wenting& Huang, Caoxin& Yang, Chen& Ge, Xilin& Huang, Wenfang& Li, Xuejun…[et al.]. Danggui Sini Decoction Protected Islet Endothelial Cell Survival from Hypoxic Damage via PI3KAkteNOS Pathway. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155529
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155529