The Chinese Medicine, Jiedu Recipe, Inhibits the Epithelial Mesenchymal Transition of Hepatocellular Carcinoma via the Regulation of Smad23 Dependent and Independent Pathways
Joint Authors
Li, Shu
Chen, Qunwei
Du, Juan
Cheng, Binbin
Lin, Wanfu
Ling, Changquan
Yin, Zifei
Zou, Yong
Liang, Shufang
Liu, Xiaolin
Zhang, Yani
Gao, Jingdong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-08
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide.
In China, traditional Chinese herb medicine has been widely used in the treatment of HCC.
Jiedu Recipe (JR) is a common used prescription which has shown good results against HCC.
However, the exact mechanisms of JR are still unknown.
Therefore, we investigated the efficacy of JR on HCC in the current study.
JR inhibited the cell viability of both SMMC-7721 and Huh7 cells in both time- and dose-dependent manners.
Transwell assay revealed that JR decreased the number of migrated cells of SMMC-7721 cells.
JR treatment increased the E-cadherin expression level and decreased the levels of p-Smad2/3 and Smad2/3.
Further study showed that JR reversed the effect of TGFβ1 on the expression of E-cadherin, vimentin, N-cadherin, and MMP2/9.
JR also significantly inhibited TGFβ1-induced migration and invasion of SMMC-7721 and Huh7 cells determined by wound healing assay and transwell assay.
TGFβ1 treatment increased the phosphorylation of Smad2/3, p38 MAPK, JNK, ERK1/2, and Akt in SMMC-7721 cells and pretreatment with JR blocked TGFβ1-induced activation of Smad2/3 and Akt and MAPKs.
In conclusion, JR inhibits liver cancer cells migration and invasion through epithelial mesenchymal transition (EMT) inhibition via Smad2/3 dependent and independent pathways, suggesting it is an effective therapeutic strategy against HCC metastasis.
American Psychological Association (APA)
Liang, Shufang& Zou, Yong& Gao, Jingdong& Liu, Xiaolin& Lin, Wanfu& Yin, Zifei…[et al.]. 2018. The Chinese Medicine, Jiedu Recipe, Inhibits the Epithelial Mesenchymal Transition of Hepatocellular Carcinoma via the Regulation of Smad23 Dependent and Independent Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1155565
Modern Language Association (MLA)
Liang, Shufang…[et al.]. The Chinese Medicine, Jiedu Recipe, Inhibits the Epithelial Mesenchymal Transition of Hepatocellular Carcinoma via the Regulation of Smad23 Dependent and Independent Pathways. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1155565
American Medical Association (AMA)
Liang, Shufang& Zou, Yong& Gao, Jingdong& Liu, Xiaolin& Lin, Wanfu& Yin, Zifei…[et al.]. The Chinese Medicine, Jiedu Recipe, Inhibits the Epithelial Mesenchymal Transition of Hepatocellular Carcinoma via the Regulation of Smad23 Dependent and Independent Pathways. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1155565
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155565