![](/images/graphics-bg.png)
Gandouling Tablets Inhibit Excessive Mitophagy in Toxic Milk (TX) Model Mouse of Wilson Disease via Pink1Parkin Pathway
Joint Authors
Zhang, Jing
Tang, Lu-Lu
Li, Liang-Yong
Cui, Shen-Wei
Jin, Shan
Chen, Huai-Zhen
Yang, Wen-Ming
Xie, Dao-Jun
Yu, Gu-Ran
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
Gandouling (GDL) tablet is a Chinese patent medicine approved by the National Medical Product Administration, which is used to treat Wilson disease (WD) in China.
In this study, we aimed to investigate the effects of GDL on mitophagy in the hippocampus in the toxic milk (TX) mouse model of WD.
Methods.
Mice were randomly divided into the following four groups: control, Wilson (model group), D-penicillamine (DPA), and GDL groups.
The animal behaviors were evaluated by the water maze experiment, traction test, and pole test.
Transmission electron microscopy was used for the detection of mitochondrion structure.
An enzyme-linked immunosorbent assay (ELISA) was performed for the analysis of the changes in liver function.
Colocalization of mitophagy-related proteins was detected by fluorescence microscopy.
Western blotting (WB) and reverse transcription-polymerase chain reaction (RT-PCR) were conducted for the detection of protein expression and mRNA levels, respectively.
Results.
Significant reduction in neurological impairments was observed in the WD model group.
All of these results were significantly reversed by GDL intervention.
Compared with the levels in the Wilson group, the levels of alanine aminotransferase (ALT), aspartate transaminase (AST), total bilirubin (TBIL), and albumin (ALB) changed obviously.
Colocalization between mitophagy-related proteins pink1, parkin, and mitochondria was changed significantly.
The mitophagy-related mRNA (pink1, parkin, and LC3II) and protein expression levels (pink1, parkin, and the rate of LC3II/LC3I) were decreased significantly, while p62 was remarkably increased after GDL intervention.
Conclusion.
Our findings indicated that the neuroprotective mechanism of GDL may occur via the inhibition of excessive mitophagy through the regulation of the pink1/parkin pathway in the TX mouse brain of WD.
American Psychological Association (APA)
Zhang, Jing& Tang, Lu-Lu& Li, Liang-Yong& Cui, Shen-Wei& Jin, Shan& Chen, Huai-Zhen…[et al.]. 2020. Gandouling Tablets Inhibit Excessive Mitophagy in Toxic Milk (TX) Model Mouse of Wilson Disease via Pink1Parkin Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1155593
Modern Language Association (MLA)
Zhang, Jing…[et al.]. Gandouling Tablets Inhibit Excessive Mitophagy in Toxic Milk (TX) Model Mouse of Wilson Disease via Pink1Parkin Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1155593
American Medical Association (AMA)
Zhang, Jing& Tang, Lu-Lu& Li, Liang-Yong& Cui, Shen-Wei& Jin, Shan& Chen, Huai-Zhen…[et al.]. Gandouling Tablets Inhibit Excessive Mitophagy in Toxic Milk (TX) Model Mouse of Wilson Disease via Pink1Parkin Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1155593
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155593