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Combination of Epicatechin 3-Gallate from Euphorbia hirta and Cefepime Promotes Potential Synergistic Eradication Action against Resistant Clinical Isolate of Pseudomonas aeruginosa
Joint Authors
Perumal, Shanmugapriya
Mohamed, Nornisah
Mahmud, Roziahanim
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Pseudomonas aeruginosa is naturally resistant to many classes of antipseudomonal antibiotics due to the species ability to easily acquire resistance.
Plant-based antibacterial agent in combination with the existing antibiotic proposes an alternative treatment regimen for the eradication of resistant bacterial infections.
The antibacterial effects of the isolated epicatechin 3-gallate compound from Euphorbia hirta in combination with cefepime were investigated in vitro against resistant P.
aeruginosa.
The fractional inhibitory concentration index of the combination was determined using checkerboard broth microdilution method.
Epicatechin 3-gallate combined with cefepime had produced synergistic effect against P.
aeruginosa (with average FIC index of 0.24).
The MIC of epicatechin 3-gallate was effectively reduced to MIC/4, MIC/8, MIC/16, and MIC/32 in the presence of cefepime.
Time-kill study of epicatechin 3-gallate combined with cefepime exhibited remarkable bactericidal activity where the eradication of P.
aeruginosa occurred within 4 h of treatment.
Scanning electron micrographs revealed apparent cell membrane damage and leakage of cytoplasmic contents from P.
aeruginosa cells which eventually led to the cell lysis after the combination treatment of epicatechin 3-gallate and cefepime.
The potential of epicatechin 3-gallate to act synergistically with cefepime against clinically resistant P.
aeruginosa strain possibly will maximize the successful outcomes when choosing empirical antibiotic treatment in hospitals or health care institutions.
American Psychological Association (APA)
Perumal, Shanmugapriya& Mahmud, Roziahanim& Mohamed, Nornisah. 2018. Combination of Epicatechin 3-Gallate from Euphorbia hirta and Cefepime Promotes Potential Synergistic Eradication Action against Resistant Clinical Isolate of Pseudomonas aeruginosa. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1155602
Modern Language Association (MLA)
Perumal, Shanmugapriya…[et al.]. Combination of Epicatechin 3-Gallate from Euphorbia hirta and Cefepime Promotes Potential Synergistic Eradication Action against Resistant Clinical Isolate of Pseudomonas aeruginosa. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1155602
American Medical Association (AMA)
Perumal, Shanmugapriya& Mahmud, Roziahanim& Mohamed, Nornisah. Combination of Epicatechin 3-Gallate from Euphorbia hirta and Cefepime Promotes Potential Synergistic Eradication Action against Resistant Clinical Isolate of Pseudomonas aeruginosa. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1155602
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155602