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Organic Extract of Justicia pectoralis Jacq. Leaf Inhibits Interferon-γ Secretion and Has Bacteriostatic Activity against Acinetobacter baumannii and Klebsiella pneumoniae
Joint Authors
Pitta, Ivan da Rocha
de Melo Rego, Moacyr Jesus Barreto
Cordeiro, Marina Ferraz
Cavalcanti, Isabella Macário Ferro
Silva, Wliana Alves Viturino da
Nunes, Tiago Rafael de Sousa
Beserra, Fernanda Gomes
Souza, Matheus Landim de
Costa-Junior, Sérgio Dias
Ferreira, Magda Rhayanny Assunção
Pitta, Maira Galdino da Rocha
Soares, Luiz Alberto Lira
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Justicia pectoralis Jacq.
(Acanthaceae) leaves currently found in the Brazilian north-east are widely used to treat diabetes, menstrual pains, asthma, and other disorders.
This work aimed to identify the phytochemical characterization and biological activities of J.
pectoralis leaf extracts.
The plant material was ground and the crude extracts were obtained with water or acetone: water (7:3 v/v), yielding aqueous (JPA), and organic (JPO) extracts.
Phytochemical characterization was performed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC).
Cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay and trypan blue (TB) exclusion assay in peripheral blood mononuclear cells (PBMCs), BALB/c splenocytes, and neoplastic cells (TOLEDO, K562, DU-145, and PANC-1) at 1, 10, and 100 μg/mL.
Antibacterial activity was evaluated using the microdilution test to obtain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).
Cytokines, IFN-γ, and IL-17A from culture supernatants of BALB/c mice splenocytes were measured by sandwich ELISA.
In the TLC analysis, both JPA and JPO extracts presented coumarin and flavonoids.
In addition, HPLC was able to identify coumarin, apigenin, and ellagic acid in both extracts.
JPO IC50 was 57.59 ± 1.03 μg/mL (MTT) and 69.44 ± 8.08 μg/mL (TB) in TOLEDO.
MIC value of JPO against Acinetobacter baumannii and Klebsiella pneumoniae was 500 μg/mL.
JPO (100 μg/mL) significantly inhibited IFN-γ levels (p=0.03).
J.
pectoralis is a potential candidate to be further investigated as an IFN-γ inhibitory agent and against Acinetobacter baumannii and Klebsiella pneumoniae.
American Psychological Association (APA)
Nunes, Tiago Rafael de Sousa& Cordeiro, Marina Ferraz& Beserra, Fernanda Gomes& Souza, Matheus Landim de& Silva, Wliana Alves Viturino da& Ferreira, Magda Rhayanny Assunção…[et al.]. 2018. Organic Extract of Justicia pectoralis Jacq. Leaf Inhibits Interferon-γ Secretion and Has Bacteriostatic Activity against Acinetobacter baumannii and Klebsiella pneumoniae. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155617
Modern Language Association (MLA)
Nunes, Tiago Rafael de Sousa…[et al.]. Organic Extract of Justicia pectoralis Jacq. Leaf Inhibits Interferon-γ Secretion and Has Bacteriostatic Activity against Acinetobacter baumannii and Klebsiella pneumoniae. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1155617
American Medical Association (AMA)
Nunes, Tiago Rafael de Sousa& Cordeiro, Marina Ferraz& Beserra, Fernanda Gomes& Souza, Matheus Landim de& Silva, Wliana Alves Viturino da& Ferreira, Magda Rhayanny Assunção…[et al.]. Organic Extract of Justicia pectoralis Jacq. Leaf Inhibits Interferon-γ Secretion and Has Bacteriostatic Activity against Acinetobacter baumannii and Klebsiella pneumoniae. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155617
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155617