In Vitro and Randomized, Double‐Blind, Placebo‐Controlled Trial to Determine the Efficacy and Safety of Nine Antiacne Medicinal Plants

Joint Authors

Saad, Bashar
Kmail, Abdalsalam
Said, Omar
Fulder, Stephen
AboFarekh, Basheer
Amin, Riyad
Daraghmeh, Jamila
Khamaysi, Iyad

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-07-09

Country of Publication

Egypt

No. of Pages

14

Main Subjects

Medicine

Abstract EN

The present in vitro and randomized, double‐blind, placebo‐controlled trial aims to determine the efficacy and safety of nine Mediterranean antiacne medicinal plants.

The antimicrobial, antisebum, and anti-inflammatory activities of the plant extracts were evaluated in cells from the immortalized human keratinocytes (HaCaT) and human monocytic cell line (THP-1) as well as in a double-blind, randomized, and placebo‐controlled trial.

Most of the extracts showed no significant cytotoxic effects on HaCaT cells up to 250 μg/ml.

Inula helenium (IH) and Saponaria officinalis (SO) inhibited sebum production at 90 μg/ml and 30 μg/ml, respectively.

The inhibition effect of SO on the growth of Cutibacterium acnes was 1.2 times higher than that of chloramphenicol.

IH and SO extracts significantly inhibited the lipopolysaccharide- (LPS-) induced IL-6 and TNF-α production in THP-1 cells reaching the control levels of untreated cells at a concentration of 250 μg/ml.

SO, IH, and Solanum nigrum (SN) extracts inhibited the nitric oxide (NO) production in a dose-dependent manner.

Based on these results, an antiacne herbal cream (AHC) was prepared from different portions of extracts from SO, IH, and SN, and its efficacy was evaluated in a double-blind, randomized, and controlled efficacy study with 41 acne patients, ages 18–24, who were asked to apply AHC (n = 27) or a placebo (n = 14) two to three times daily for six weeks.

Results obtained indicate that the AHC has unique synergistic effects that halt sebum production, combined with highly antiseptic and anti-inflammatory activity, in which 54.95% (t = 19.37 P<0.001) of acne inflammatory and noninflammatory lesions disappeared after two weeks, 85.3%, after five weeks (t = 14.19 P<0.001), and 91.4%, at the end of the sixth week of application (t = 5.7 P<0.001).

In conclusion, SO, IH, and SN as single extracts and in combination as AHC showed significant antimicrobial, antisebum, and anti-inflammatory activities in vitro and in a double-blind, randomized, and controlled antiacne efficacy.

Therefore, AHC represents an interesting alternative treatment for acne.

American Psychological Association (APA)

Said, Omar& Khamaysi, Iyad& Kmail, Abdalsalam& Fulder, Stephen& AboFarekh, Basheer& Amin, Riyad…[et al.]. 2020. In Vitro and Randomized, Double‐Blind, Placebo‐Controlled Trial to Determine the Efficacy and Safety of Nine Antiacne Medicinal Plants. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1155637

Modern Language Association (MLA)

Said, Omar…[et al.]. In Vitro and Randomized, Double‐Blind, Placebo‐Controlled Trial to Determine the Efficacy and Safety of Nine Antiacne Medicinal Plants. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1155637

American Medical Association (AMA)

Said, Omar& Khamaysi, Iyad& Kmail, Abdalsalam& Fulder, Stephen& AboFarekh, Basheer& Amin, Riyad…[et al.]. In Vitro and Randomized, Double‐Blind, Placebo‐Controlled Trial to Determine the Efficacy and Safety of Nine Antiacne Medicinal Plants. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1155637

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1155637