Herb Formula ZhenRongDan Balances Sex Hormones, Modulates Organ Atrophy, and Restores ERα and ERβ Expressions in Ovariectomized Rats
Joint Authors
Zhang, Xuan
Chen, Qian
Chen, Bo
Wang, Fangqin
Chen, Xiao-Hong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Herb mixtures are widely used for treatment of the menopausal syndrome long before the hormonal therapy.
However, there is insufficient data for herb remedies in treating menopausal syndromes.
Here we aim to investigate the effect of ZhenRongDan (ZRD) in balancing female hormones, regulating expression of estrogen receptors (ERs), and preventing organ atrophy in menopausal rats.
Rats that underwent bilateral ovariectomy were used in the experiments; the effects of ZRD on serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and estradiol (E2) levels were observed.
Histology of vagina and ERs expression in vagina, uterus, and adrenal gland were also examined.
ELISAs were used to analyze the changes of FSH, LH, PRL, and E2 in serum, and the morphological changes of the cervical epithelium cells were observed by Hematoxylin & Eosin (H&E) staining.
Immunohistochemistry and western blot were applied to detect estrogen receptors subtypes alpha (ERα) and beta (ERβ) expression in vagina, uterus, and adrenal gland.
We found that ZRD could significantly reduce the weight of the adrenal gland and increase the weight of the uterus.
It could decrease the release of FSH and LH as well as increasing E2 and PRL levels.
Furthermore, ZRD could improve the number of cervical vaginal epithelial cells and increase the thickness of the vaginal wall.
And the altered expressions of ERα and ERβ are also restored by ZRD.
ZRD could obviously relieve the endocrine disorders, modulate organ atrophy, and restore ERα and ERβ expression in the ovariectomized rat model.
American Psychological Association (APA)
Zhang, Xuan& Chen, Qian& Chen, Bo& Wang, Fangqin& Chen, Xiao-Hong. 2018. Herb Formula ZhenRongDan Balances Sex Hormones, Modulates Organ Atrophy, and Restores ERα and ERβ Expressions in Ovariectomized Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155667
Modern Language Association (MLA)
Zhang, Xuan…[et al.]. Herb Formula ZhenRongDan Balances Sex Hormones, Modulates Organ Atrophy, and Restores ERα and ERβ Expressions in Ovariectomized Rats. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1155667
American Medical Association (AMA)
Zhang, Xuan& Chen, Qian& Chen, Bo& Wang, Fangqin& Chen, Xiao-Hong. Herb Formula ZhenRongDan Balances Sex Hormones, Modulates Organ Atrophy, and Restores ERα and ERβ Expressions in Ovariectomized Rats. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155667
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155667