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Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense
Joint Authors
Lago, João Henrique G.
Zagmignan, Adrielle
Gonçalves, Letícia Machado
de Sousa, Eduardo Martins
Lima Viana, José
Lima Lobato, Luís Felipe
Gomes Abreu, Afonso
Sá, Joicy Cortez de
Monteiro, Cristina de Andrade
Carvalho, Rafael Cardoso
Neto, Lídio Gonçalves Lima
Nascimento da Silva, Luís Cláudio
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The incidence of infections caused by rapidly growing mycobacteria (RGM), especially Mycobacterium abscessus subsp.
massiliense (Mabs), is increasing worldwide.
Severe infections are associated with abscess formation and strong inflammatory response.
This study evaluated the antimicrobial and anti-inflammatory activities of a hydroalcoholic extract (BoHE) and ethyl acetate fraction (BoEA) of Bixa orellana leaves.
Antimicrobial activity was evaluated by broth microdilution to determine the minimum inhibitory (MIC) and the minimum bactericidal (MBC) concentrations.
Cytotoxicity was evaluated using erythrocytes and RAW 264.7 cells.
Nitric oxide (NO) was assayed in stimulated RAW 264.7 cells, and inflammatory cell migration and acute toxicity were evaluated in a Mabs-induced peritonitis mouse model.
The compounds present in BoEA were identified by high performance liquid chromatography and mass spectrometry (HPLC-MS).
The MIC and MBC values were 2.34 mg/mL and 37.5 mg/mL for BoHE and 0.39 mg/mL and 6.25 mg/mL for BoEA.
The extracts did not induce significant toxicity in erythrocytes and RAW 264.7 cells.
High levels of NO induced by Mabs were decreased by treatment with both extracts.
The anti-inflammatory activity was confirmed in vivo by significant reduction of the cell migration to the peritoneum following BoHE and BoEA pretreatment.
Animals treated with BoHE or BoEA did not show signs of acute toxicity in stomach, liver, and kidney.
The chemical characterization of BoEA (the most active extract) revealed that kaempferol-3-O-coumaroyl glucose is its major component.
The extract of B.
orellana may be effective for treating infections caused by Mabs.
American Psychological Association (APA)
Lima Viana, José& Zagmignan, Adrielle& Lima Lobato, Luís Felipe& Gomes Abreu, Afonso& Nascimento da Silva, Luís Cláudio& Sá, Joicy Cortez de…[et al.]. 2018. Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1155751
Modern Language Association (MLA)
Lima Viana, José…[et al.]. Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1155751
American Medical Association (AMA)
Lima Viana, José& Zagmignan, Adrielle& Lima Lobato, Luís Felipe& Gomes Abreu, Afonso& Nascimento da Silva, Luís Cláudio& Sá, Joicy Cortez de…[et al.]. Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1155751
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155751