Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia
Joint Authors
Zhang, Shengsheng
Wu, Zhenyu
Lu, Xiaofang
Zhu, Chunyang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The present study investigated the effect of Chinese medicine Sini-San (SNS) on visceral hypersensitivity in a rat model of functional dyspepsia (FD), and it explored related underlying mechanisms.
The rat model of FD was developed by combining neonatal iodoacetamide (IA) treatment and adult tail-clamping.
After SNS treatment, the behavior and electromyographic testing were performed to evaluate the visceromotor responses of rats to gastric distention.
Immunofluorescence was used to detect the distribution of iNOS-positive cells in the spinal dorsal horn, while the real-time quantitative PCR and western blot were used for detection of the gene expression of c-fos, iNOS, and GABAb and protein levels of iNOS and GABAb in the spinal dorsal horn, respectively.
The protein concentration of cGMP and PKG proteins in the spinal dorsal horn were quantified by enzyme-linked immunosorbent assay.
In this study, SNS treatment significantly reduced the behavioral score and electromyographic response to graded intragastric distension pressure.
The middle-dose of SNS treatment significantly reduced the distribution of iNOS-positive cells in the spinal dorsal horn of FD model rats.
The gene expression of c-fos, iNOS, and GABAb and the protein contents of iNOS, GABAb, cGMP, and PKG in the spinal dorsal horn of FD model rats were restored to a normal level by middle-dose of SNS treatment.
Our results suggest that Sini-San may alleviate the visceral hypersensitivity in FD model rats via regulation of the NO/cGMP/PKG pathway in the spinal dorsal horn.
American Psychological Association (APA)
Wu, Zhenyu& Lu, Xiaofang& Zhang, Shengsheng& Zhu, Chunyang. 2020. Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1155780
Modern Language Association (MLA)
Wu, Zhenyu…[et al.]. Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1155780
American Medical Association (AMA)
Wu, Zhenyu& Lu, Xiaofang& Zhang, Shengsheng& Zhu, Chunyang. Sini-San Regulates the NO-cGMP-PKG Pathway in the Spinal Dorsal Horn in a Modified Rat Model of Functional Dyspepsia. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1155780
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155780