Smilax aristolochiifolia Root Extract and Its Compounds Chlorogenic Acid and Astilbin Inhibit the Activity of α-Amylase and α-Glucosidase Enzymes
Joint Authors
Gutiérrez-Uribe, Janet A.
Lugo-Cervantes, Eugenia
Pérez-Nájera, Viridiana Candelaria
Antunes-Ricardo, Marilena
Hidalgo-Figueroa, Sergio
Del-Toro-Sánchez, Carmen Lizette
Salazar-Olivo, Luis A.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Regulating activities of α-amylase and α-glucosidase through the use of specific inhibitors is a main strategy for controlling type 2 diabetes.
Smilax aristolochiifolia root decoctions are traditionally used in Mexico as hypoglycemic and for weight loss, but the active principles and mechanisms underlying such putative metabolic effects are yet unknown.
Here, we isolated the major bioactive compounds from a hydroethanolic extract of S.
aristolochiifolia root by fast centrifugal partition chromatography and evaluated their effects against pancreatic α-amylase and yeast α-glucosidase.
A chlorogenic acid-rich fraction (CAF) inhibited α-amylase activity with an IC50 value of 59.28 μg/mL in an uncompetitive manner and α-glucosidase activity with an IC50 value of 9.27 μg/mL in a noncompetitive mode.
Also, an astilbin-rich fraction (ABF) inhibited α-glucosidase activity with an IC50 value of 12.30 μg/mL, in a noncompetitive manner.
CAF inhibition α-amylase was as active as acarbose while both CAF and ABF were 50-fold more potent inhibitors of α-glucosidase than acarbose.
The molecular docking results of chlorogenic acid and astilbin with α-amylase and α-glucosidase enzymes correlated with the inhibition mechanisms suggested by enzymatic assays.
Our results prove that S.
aristolochiifolia roots contain chlorogenic acid and astilbin, which inhibit carbohydrates-hydrolyzing enzymes, suggesting a new mechanism for the hypoglycemic effect reported for this plant.
American Psychological Association (APA)
Pérez-Nájera, Viridiana Candelaria& Gutiérrez-Uribe, Janet A.& Antunes-Ricardo, Marilena& Hidalgo-Figueroa, Sergio& Del-Toro-Sánchez, Carmen Lizette& Salazar-Olivo, Luis A.…[et al.]. 2018. Smilax aristolochiifolia Root Extract and Its Compounds Chlorogenic Acid and Astilbin Inhibit the Activity of α-Amylase and α-Glucosidase Enzymes. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1155820
Modern Language Association (MLA)
Pérez-Nájera, Viridiana Candelaria…[et al.]. Smilax aristolochiifolia Root Extract and Its Compounds Chlorogenic Acid and Astilbin Inhibit the Activity of α-Amylase and α-Glucosidase Enzymes. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1155820
American Medical Association (AMA)
Pérez-Nájera, Viridiana Candelaria& Gutiérrez-Uribe, Janet A.& Antunes-Ricardo, Marilena& Hidalgo-Figueroa, Sergio& Del-Toro-Sánchez, Carmen Lizette& Salazar-Olivo, Luis A.…[et al.]. Smilax aristolochiifolia Root Extract and Its Compounds Chlorogenic Acid and Astilbin Inhibit the Activity of α-Amylase and α-Glucosidase Enzymes. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1155820
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155820