An LC-MSMS Method for Simultaneous Determination of the Toxic and Active Components of Cortex Periplocae in Rat Plasma and Application to a Pharmacokinetic Study
Joint Authors
Chang, Yanxu
Liu, Rui
Li, Jin
He, Jun
Hao, Jia
Li, Zhen
Li, Yang
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A sensitive and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneously determine the toxic and other active components including isovanillin, scopoletin, periplocin, periplogenin, and periplocymarin after oral administration of cortex periplocae extract to rats.
Plasma samples were prepared by protein precipitation with methanol.
All compounds were separated on a C18 column with gradient elution using acetonitrile and formic acid aqueous solution (0.1%, v/v) as the mobile phase at a flow rate of 0.3 mL/min.
The detection of all compounds was accomplished by multiple-reaction monitoring (MRM) in the positive electrospray ionization mode.
The LC-MS/MS method exhibited good linearity for five analytes.
The lower limit of quantification (LLOQ) was 0.48 ng/mL for scopoletin, periplogenin, and periplocymarin; 2.4 ng/mL for isovanillin and periplocin.
The extraction recoveries of all compounds were more than 90% and the RSDs were below 10%.
It was found that the absorption of scopoletin and periplocin was rapid in vivo after oral administration of cortex periplocae extract.
Furthermore, periplocymarin possessed abundant plasma exposure.
The results demonstrated that the validated method was efficiently applied for the pharmacokinetic studies of isovanillin, scopoletin, periplocin, periplogenin, and periplocymarin after oral administration of cortex periplocae extract.
American Psychological Association (APA)
Li, Zhen& Li, Yang& Li, Jin& Liu, Rui& Hao, Jia& He, Jun…[et al.]. 2019. An LC-MSMS Method for Simultaneous Determination of the Toxic and Active Components of Cortex Periplocae in Rat Plasma and Application to a Pharmacokinetic Study. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1155833
Modern Language Association (MLA)
Li, Zhen…[et al.]. An LC-MSMS Method for Simultaneous Determination of the Toxic and Active Components of Cortex Periplocae in Rat Plasma and Application to a Pharmacokinetic Study. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1155833
American Medical Association (AMA)
Li, Zhen& Li, Yang& Li, Jin& Liu, Rui& Hao, Jia& He, Jun…[et al.]. An LC-MSMS Method for Simultaneous Determination of the Toxic and Active Components of Cortex Periplocae in Rat Plasma and Application to a Pharmacokinetic Study. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1155833
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155833