Development and Validation of an HPLC Method for Simultaneous Assay of MCI and MI in Shampoos Containing Plant Extracts
Joint Authors
Thi Huong Hoa, Le
Tran Ngoc Hung, Vo
Thu Trang, Do
Nguyen Hung Thu, Thai
Le, Dinh Chi
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A simple, easy-to-implement HPLC method was developed and validated for simultaneous determination of two isothiazolinone preservatives, methylchloroisothiazolinone (MCI) and methylisothiazolinone (MI), in hair care shampoo containing plant extracts.
In this method, shampoo samples were first dissolved in isopropyl myristate and then MCI and MI were extracted from isopropyl myristate layer by a mixture of methanol and 0.02 M phosphate buffer solution pH 3.0 (30: 70, v/v) and analyzed on an analytical biphenyl column maintained at 25°C with a mixture of methanol and water (10: 90, v/v) in isocratic elution mode as mobile phase.
Total flow rate of mobile phase was maintained at 1.0 mL per minute.
The UV detection was performed at 274 nm.
Injection volume was 50 μl.
The method was fully validated in terms of specificity, linearity, precision, accuracy, and robustness according to requirements of AOAC International and was proved as reliable and suitable for the intended application.
American Psychological Association (APA)
Thi Huong Hoa, Le& Tran Ngoc Hung, Vo& Thu Trang, Do& Nguyen Hung Thu, Thai& Le, Dinh Chi. 2019. Development and Validation of an HPLC Method for Simultaneous Assay of MCI and MI in Shampoos Containing Plant Extracts. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1155842
Modern Language Association (MLA)
Thi Huong Hoa, Le…[et al.]. Development and Validation of an HPLC Method for Simultaneous Assay of MCI and MI in Shampoos Containing Plant Extracts. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1155842
American Medical Association (AMA)
Thi Huong Hoa, Le& Tran Ngoc Hung, Vo& Thu Trang, Do& Nguyen Hung Thu, Thai& Le, Dinh Chi. Development and Validation of an HPLC Method for Simultaneous Assay of MCI and MI in Shampoos Containing Plant Extracts. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1155842
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155842