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Simultaneous Determination of Schisandrin and Promethazine with Its Metabolite in Rat Plasma by HPLC-MSMS and Its Application to a Pharmacokinetic Study
Joint Authors
Zhang, Ping
Zhao, Yanling
Gao, Sijia
Zhou, Xuelin
Lang, Liwei
Liu, Honghong
Li, Jianyu
Li, Haotian
Wang, Dan
Xu, Zhuo
Cai, Huadan
Zou, Wenjun
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
This study aimed to develop a selective, simple, and sensitive HPLC-MS/MS method for the simultaneous determination of schisandrin and promethazine (PMZ) with its metabolite in rat plasma, which was further used for a pharmacokinetic herb-drug interaction study.
HPLC-MS/MS analyses were performed on an Agilent Technologies 1290 LC and a 6410 triple quadrupole mass spectrometer.
The following parameters, the lower limit of quantification (LLOQ), calibration curve, accuracy, precision, stability, matrix effect, and recovery, were validated.
The linear range of the developed method for PMZ, its metabolite promethazine sulfoxide (PMZSO), and schisandrin in rat plasma was 0.5–200 ng/mL (R2 > 0.995), with an LLOQ of 0.5 ng/mL, which completely met the determination requirements of biosamples.
The intra- and interday precision (RSD, %) was below 13.31% (below 16.67% for the LLOQ) in various plasma, whose accuracy (bias, %) was from −8.52% to 11.40%, which were both within an acceptable range.
This method was successfully applied to a pharmacokinetic herb-drug interaction study after oral administration of PMZ with or without S.
chinensis water extract.
The results demonstrated that coadministration with the S.
chinensis water extract might affect the pharmacokinetic behaviors of PMZ.
In turn, when taken together with PMZ, the pharmacokinetic parameters of schisandrin, the main active component of S.
chinensis, were also affected.
The method established in the current study was selective, simple, sensitive, and widely available with good linearity, high accuracy and precision, and a stable sample preparation process.
Moreover, this analytical method provides a significant approach for the investigation of herb-drug interaction between S.
chinensis and PMZ.
The potential pharmacokinetic herb-drug interaction of PMZ- and schisandrin-containing preparations should be noted.
American Psychological Association (APA)
Gao, Sijia& Zhou, Xuelin& Lang, Liwei& Liu, Honghong& Li, Jianyu& Li, Haotian…[et al.]. 2019. Simultaneous Determination of Schisandrin and Promethazine with Its Metabolite in Rat Plasma by HPLC-MSMS and Its Application to a Pharmacokinetic Study. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1155871
Modern Language Association (MLA)
Gao, Sijia…[et al.]. Simultaneous Determination of Schisandrin and Promethazine with Its Metabolite in Rat Plasma by HPLC-MSMS and Its Application to a Pharmacokinetic Study. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1155871
American Medical Association (AMA)
Gao, Sijia& Zhou, Xuelin& Lang, Liwei& Liu, Honghong& Li, Jianyu& Li, Haotian…[et al.]. Simultaneous Determination of Schisandrin and Promethazine with Its Metabolite in Rat Plasma by HPLC-MSMS and Its Application to a Pharmacokinetic Study. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1155871
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155871