Fast and Simple LC-MSMS Method for Rifampicin Quantification in Human Plasma
Joint Authors
Temova Rakuša, Žane
Roškar, Robert
Klančar Andrejc, Anita
Trdan Lušin, Tina
Faganeli, Nataša
Grabnar, Iztok
Mrhar, Aleš
Kristl, Albin
Trontelj, Jurij
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A simple, fast, and cost-effective LC-MS/MS method for quantification of rifampicin in human plasma was developed and fully validated.
The plasma samples containing rifampicin and isotopically labelled internal standard rifampicin D8, were cleaned up using a Captiva ND Lipids filtration plate.
Chromatographic separation was achieved on an 1290 Infinity liquid chromatograph coupled to 6460 Triple Quadrupole operated in positive mode on a core-shell Kinetex C18 column (50 × 2.1 mm, 2.6 μm) by gradient elution using 0.1% formic acid in water and acetonitrile as a mobile phase.
The proposed method is the fastest method published by now, both in terms of sample preparation (approximately one minute per sample) and chromatographic analysis (total run time 2.4 min).
Another key benefit is the outstanding sensitivity and wide analytical range (5-40000 μg/L) with good linearity, accuracy, and precision.
The method showed almost complete recovery (92%) and absence of any significant matrix effect as demonstrated by uniform responses from QC samples prepared in blood plasma from 6 volunteers (RSD <5%).
The proposed method was successfully applied to rifampicin quantification in 340 patients’ plasma samples, thus demonstrating its suitability for both therapeutic drug monitoring and pharmacokinetic analysis.
American Psychological Association (APA)
Temova Rakuša, Žane& Roškar, Robert& Klančar Andrejc, Anita& Trdan Lušin, Tina& Faganeli, Nataša& Grabnar, Iztok…[et al.]. 2019. Fast and Simple LC-MSMS Method for Rifampicin Quantification in Human Plasma. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1155891
Modern Language Association (MLA)
Temova Rakuša, Žane…[et al.]. Fast and Simple LC-MSMS Method for Rifampicin Quantification in Human Plasma. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1155891
American Medical Association (AMA)
Temova Rakuša, Žane& Roškar, Robert& Klančar Andrejc, Anita& Trdan Lušin, Tina& Faganeli, Nataša& Grabnar, Iztok…[et al.]. Fast and Simple LC-MSMS Method for Rifampicin Quantification in Human Plasma. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1155891
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155891