Absorption Characteristics of Combination Medication of Realgar and Indigo Naturalis: In Vitro Transport across MDCK-MDR1 Cells and In Vivo Pharmacokinetics in Mice after Oral Administration
Joint Authors
Yin, Xingbin
Ni, Jian
Zhang, Miao
Guo, Lin
Lin, Long-Fei
Qu, Chang-Hai
Luo, Shi-Lin
Zhang, Xin
Zhang, Hai-Ying
Liang, Xiao
Guan, Jun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Realgar and indigo naturalis are clinically combined to treat varieties of leukemia.
Exploring the drug-drug interactions might be beneficial to find active substances and develop new targeted drugs.
This study aimed at exploring the change of arsenic concentration in mice and across MDCK-MDR1 cells and the cytotoxicity on K562 cells when realgar and indigo naturalis were combined.
In the presence or absence of indigo naturalis, pharmacokinetics and cell-based permeability assays were used to evaluate the change of arsenic concentration, and K562 cell line was applied to evaluate the change of cytotoxicity.
The drug concentration-time profiles exhibited that the combination medication group generated higher AUC, thalf, and longer MRT for arsenic, compared with the single administration of realgar.
The apparent permeability coefficients (Papp) of bidirectional transport in MDCK-MDR1 cell permeability experiments showed that arsenic permeability obviously went up when indigo naturalis was incubated together.
The combination medication significantly decreased the cell viability of K562 cells when both the concentration of realgar and the concentration of indigo naturalis were nontoxic.
The pharmacokinetic research, the MDCK-MDR1 based permeability study, and the K562 cytotoxicity study were united together to verify the combination medication of realgar and indigo naturalis enhanced the absorption and the permeability across cells for arsenic and effectively inhibited the proliferation of K562 cell line.
The molecular binding of As4S4 and indirubin was analyzed by computational study.
It is predicted that the formation of the complex [As4S4…Indirubin] involves noncovalent interaction that changes the concentration of arsenic.
American Psychological Association (APA)
Zhang, Miao& Guo, Lin& Lin, Long-Fei& Qu, Chang-Hai& Yin, Xingbin& Luo, Shi-Lin…[et al.]. 2018. Absorption Characteristics of Combination Medication of Realgar and Indigo Naturalis: In Vitro Transport across MDCK-MDR1 Cells and In Vivo Pharmacokinetics in Mice after Oral Administration. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155903
Modern Language Association (MLA)
Zhang, Miao…[et al.]. Absorption Characteristics of Combination Medication of Realgar and Indigo Naturalis: In Vitro Transport across MDCK-MDR1 Cells and In Vivo Pharmacokinetics in Mice after Oral Administration. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1155903
American Medical Association (AMA)
Zhang, Miao& Guo, Lin& Lin, Long-Fei& Qu, Chang-Hai& Yin, Xingbin& Luo, Shi-Lin…[et al.]. Absorption Characteristics of Combination Medication of Realgar and Indigo Naturalis: In Vitro Transport across MDCK-MDR1 Cells and In Vivo Pharmacokinetics in Mice after Oral Administration. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1155903
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155903