Immunomodulation Effects of Schizonepeta tenuifolia Briq. on the IgE-Induced Allergic Model of RBL-2H3 Cells
Joint Authors
Lin, Yi-Hsuan
Chen, Hsing-Yu
Yang, Sien-Hung
Chiu, Jung-Chun
Chen, Kun-Jei
Ho, Hung-Yao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Schizonepeta tenuifolia (ST) Briq.
is a traditional herbal medicine commonly used to treat allergic skin diseases, where the inflammation process is closely related to symptom severity.
This study aimed to explore the immunomodulatory effect of ST by using immunoglobulin E- (IgE-) stimulated RBL-2H3 cell cultures, a common cell line for studying mast cell degranulation and inflammatory cytokine release in vitro.
After stimulating the RBL-2H3 cells with IgE, ST at concentrations of 10, 50, or 100 μg/mL was added to the cell cultures.
Cell viability, inflammatory cytokines (IL-6, IL-13, IL-4, TNF-α, and IFN-γ), anti-inflammatory cytokine IL-10, and degranulation ability were examined 48 and 72 hours after administration of ST.
The markers of inflammation and allergic reaction, IFN-γ, TNF-α, IL-4, and IL-6, were suppressed, especially after treatment with 100 μg/mL ST.
However, the anti-inflammation marker IL-10 was also suppressed by ST.
Trend analysis showed that a higher ST concentration was associated with lower IFN-γ and TNF-α levels.
Moreover, degranulation of RBL-2H3 cells was assessed by measuring the release of β-hexosaminidase, which was suppressed by ST at 10 μg/mL.
This study showed an immunomodulatory effect of ST at the cellular level and suggests the role of ST in treating allergic diseases.
American Psychological Association (APA)
Lin, Yi-Hsuan& Chen, Hsing-Yu& Chiu, Jung-Chun& Chen, Kun-Jei& Ho, Hung-Yao& Yang, Sien-Hung. 2018. Immunomodulation Effects of Schizonepeta tenuifolia Briq. on the IgE-Induced Allergic Model of RBL-2H3 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1155918
Modern Language Association (MLA)
Lin, Yi-Hsuan…[et al.]. Immunomodulation Effects of Schizonepeta tenuifolia Briq. on the IgE-Induced Allergic Model of RBL-2H3 Cells. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1155918
American Medical Association (AMA)
Lin, Yi-Hsuan& Chen, Hsing-Yu& Chiu, Jung-Chun& Chen, Kun-Jei& Ho, Hung-Yao& Yang, Sien-Hung. Immunomodulation Effects of Schizonepeta tenuifolia Briq. on the IgE-Induced Allergic Model of RBL-2H3 Cells. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1155918
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155918