Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography
Joint Authors
Marchetti, Nicola
Giovannini, Pier Paolo
Catani, Martina
Pasti, Luisa
Cavazzini, Alberto
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The retention mechanism of four major carotenoids, two xanthophylls (i.e., lutein and zeaxanthin) and two carotenes (i.e., lycopene and β-carotene), was investigated in reversed-phase liquid chromatography with the aim of thermodynamic analysis.
The experimental variables considered in this study were the composition of mobile phase (MP) and the temperature.
Chromatographic elutions were undertaken under linear, isocratic conditions by using a C18 stationary phase, four different MP compositions (by varying the ratio methanol/acetonitrile from 66.5/28.5 to 47.5/47.5 v/v), and column temperatures in the range 283–313 K.
Traditional Van’t Hoff analysis has been used to estimate changes of standard enthalpy (ΔH∘) and Gibbs free energy (ΔG∘) associated with the solute transfer from the mobile to the stationary phase at each mobile phase composition.
The thermodynamic quantities have been correlated to the structure of investigated carotenoids and their interaction with the octadecyl silica stationary phase.
American Psychological Association (APA)
Marchetti, Nicola& Giovannini, Pier Paolo& Catani, Martina& Pasti, Luisa& Cavazzini, Alberto. 2019. Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1155965
Modern Language Association (MLA)
Marchetti, Nicola…[et al.]. Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1155965
American Medical Association (AMA)
Marchetti, Nicola& Giovannini, Pier Paolo& Catani, Martina& Pasti, Luisa& Cavazzini, Alberto. Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1155965
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155965