Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity
Joint Authors
Chen, Su-Hong
Lv, Gui-Yuan
Cai, Xia-Miao
Qian, Liu-Qing
Chen, Xue
Yang, Ke
Lu, Ting-Ting
Zhan, Liang-Hui
Lu, Haixia
Zhou, Cong
Li, He
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In order to establish the extraction technology of flavonoids from Dendrobium officinale leaves, a method combining Plackett–Burman design (PBD), steepest ascent design, and central composite design was developed to optimize the extraction of flavonoids.
In addition, the tyrosinase activity inhibition of flavonoids was further tested in vitro.
PBD results showed that ethanol concentration and number of extractions were key factors.
Response surface methodology (RSM) indicated that the optimal extraction conditions were 78% ethanol concentration, six extraction times, 2 h, and 1:50 solid-liquid ratio.
Under these conditions, the total flavonoid content could reach 35 mg/50 mL.
In vitro tyrosinase experiment, the extracted total flavonoids had better inhibitory effect on tyrosinase activity than β-arbutin, and its inhibition rate for monophenolase and diphenolase exceeded 100% and 70%, respectively.
These results indicate that RSM can effectively improve the extraction of flavonoids from Dendrobium officinale leaves and the flavonoids have the prospect of being applied to foods and cosmetics.
American Psychological Association (APA)
Lu, Haixia& Yang, Ke& Zhan, Liang-Hui& Lu, Ting-Ting& Chen, Xue& Cai, Xia-Miao…[et al.]. 2019. Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1155978
Modern Language Association (MLA)
Lu, Haixia…[et al.]. Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1155978
American Medical Association (AMA)
Lu, Haixia& Yang, Ke& Zhan, Liang-Hui& Lu, Ting-Ting& Chen, Xue& Cai, Xia-Miao…[et al.]. Optimization of Flavonoid Extraction in Dendrobium officinale Leaves and Their Inhibitory Effects on Tyrosinase Activity. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1155978
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155978