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Preparative Separation of Three Monoterpenes from Perilla frutescens var. crispa Using Centrifugal Partition Chromatography
Joint Authors
Nam, Bomi
Paudel, Sunil Babu
Kim, Jin-Baek
Jin, Chang Hyun
Lee, Dongho
Nam, Joo-Won
Han, Ah-Reum
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-09
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Three monoterpenes, namely, 9-hydroxy isoegomaketone (1), isoegomaketone (2), and perilla ketone (3), were successfully separated from the supercritical carbon dioxide (SC-CO2) extract of the leaves of Perilla frutescens var.
crispa (cv.
Antisperill; Lamiaceae) by centrifugal partition chromatography (CPC).
To obtain large quantities of these materials required for studies on their mechanism of action and in vivo effectiveness in inflammation, we used CPC because of its high loading capacity and reproducibility to purify the three compounds.
Compound 1 (2.60 mg, 96.7% purity at 254 nm) was purified from 500 mg of the SC-CO2 extract of P.
frutescens var.
crispa (cv.
Antisperill), using a two-phase solvent system comprising n-hexane/ethyl acetate/ethanol/water (5:5:5:5 v/v) in a descending mode.
As compounds 2 (56.1 mg, 97.6% purity at 254 nm) and 3 (78.6 mg, 96.1% purity at 254 nm) are highly volatile and difficult to recover from an aqueous mobile phase after purification during the drying process, they were obtained from the same amount of the processed extract in an ascending mode using the upper organic phase as the mobile phase (n-hexane/ethyl acetate/ethanol/water, 8:2:8:2 v/v).
The structures of compounds 1–3 were confirmed by 1H- and 13C-NMR analysis.
Thus, based on our findings, we recommend centrifugal partition chromatography as a powerful technique for purifying the active principal compounds 1 and 2 from the leaves of P.
frutescens var.
crispa.
American Psychological Association (APA)
Nam, Bomi& Paudel, Sunil Babu& Kim, Jin-Baek& Jin, Chang Hyun& Lee, Dongho& Nam, Joo-Won…[et al.]. 2019. Preparative Separation of Three Monoterpenes from Perilla frutescens var. crispa Using Centrifugal Partition Chromatography. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1155992
Modern Language Association (MLA)
Nam, Bomi…[et al.]. Preparative Separation of Three Monoterpenes from Perilla frutescens var. crispa Using Centrifugal Partition Chromatography. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1155992
American Medical Association (AMA)
Nam, Bomi& Paudel, Sunil Babu& Kim, Jin-Baek& Jin, Chang Hyun& Lee, Dongho& Nam, Joo-Won…[et al.]. Preparative Separation of Three Monoterpenes from Perilla frutescens var. crispa Using Centrifugal Partition Chromatography. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1155992
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155992