Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis and Necroptosis via Inhibiting Endoplasmic Reticulum Stress
Joint Authors
Wang, Baichuan
Shao, Zengwu
Wang, Wenzheng
Qing, Xiangcheng
Ma, Kaige
Wei, Yulong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Tauroursodeoxycholic acid (TUDCA) is a kind of hydrophilic bile acid, which could protect cells from death via inhibiting endoplasmic reticulum (ER) stress.
However, the role of TUDCA in compression-induced intervertebral disc degeneration (IVDD) has not been elucidated.
Here, we used a previously described device to mimic in vivo compression conditions.
NP cells treated with DMSO or TUDCA were exposed to compression.
Then, cell viability, morphology, and apoptosis were detected.
Furthermore, apoptosis-related proteins and necroptosis markers were detected too.
To investigate the specific cytoprotective mechanisms of TUDCA in IVDD, we detected the ER morphology by electron microscopy.
In addition, the ER stress of nucleus pulposus (NP) cells was quantitatively evaluated by analyzing the level of ER-stress-associated proteins.
Our results revealed that TUDCA could protect NP cells from excessive compression-induced death by reducing the apoptosis and necroptosis.
In addition, ER stress is involved in pathogenesis of IVDD induced by excessive compression and plays a detrimental role.
TUDCA exerts its protective functions by inhibiting ER stress.
In conclusion, TUDCA could protect NP cells from compression-induced death, which suggested that treatment by TUDCA may be a potential method to retard IVDD.
American Psychological Association (APA)
Wang, Wenzheng& Qing, Xiangcheng& Wang, Baichuan& Ma, Kaige& Wei, Yulong& Shao, Zengwu. 2018. Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis and Necroptosis via Inhibiting Endoplasmic Reticulum Stress. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1155994
Modern Language Association (MLA)
Wang, Wenzheng…[et al.]. Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis and Necroptosis via Inhibiting Endoplasmic Reticulum Stress. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1155994
American Medical Association (AMA)
Wang, Wenzheng& Qing, Xiangcheng& Wang, Baichuan& Ma, Kaige& Wei, Yulong& Shao, Zengwu. Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis and Necroptosis via Inhibiting Endoplasmic Reticulum Stress. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1155994
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155994