Green Analytical Methods for the Separation of Seven Antihistamines: Application in Separation of Azelastine and Related Impurities in Nasal Solution
Joint Authors
da Costa, Lucas Maciel
Oliveira de Almeida Leite, Heitor
Kassab, Nájla Mohamad
Singh, Anil Kumar
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-11
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Antihistamines are widely used to alleviate the symptoms caused by allergic reactions.
Most of these drugs have zwitteriónicas and/or amphoteric characteristics, which confer additional analytical challenges.
This work aimed to develop a single eco-friendly and efficient chromatographic methods for analysis of seven antihistamines, namely, azelastine HCl, desloratadine, ebastine, fexofenadine HCl, ketotifen, loratadine, and olopatadine HCl.
The separations were obtained using RP C-18 LUNA (150x4.6mm, 5 μm) column.
The mobile phase consisted of acetonitrile and acidified water (pH 2.1) in the following proportion: 15:85, v/v for desloratadine, 25:75, v/v for ketotifen and olopatadine, 32:68, v/v for fexofenadine, 35:65, v/v for azelastine and loratadine, and 45:55, v/v for ebastine.
All separations were obtained in less than 7.0 min.
A prototype method was fully validated and applied in the assay of azelastine HCl in nasal solutions.
The proposed methods for analysis of seven antihistamines are highly efficient, selective, and sensitive.
Moreover, all methods can be considered excellent in terms of greenness, with total organic residue < 2.5 mL/analysis.
An improved gradient method is also described for separation of azelastine HCl and its related impurities.
American Psychological Association (APA)
da Costa, Lucas Maciel& Oliveira de Almeida Leite, Heitor& Kassab, Nájla Mohamad& Singh, Anil Kumar. 2019. Green Analytical Methods for the Separation of Seven Antihistamines: Application in Separation of Azelastine and Related Impurities in Nasal Solution. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1156007
Modern Language Association (MLA)
da Costa, Lucas Maciel…[et al.]. Green Analytical Methods for the Separation of Seven Antihistamines: Application in Separation of Azelastine and Related Impurities in Nasal Solution. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1156007
American Medical Association (AMA)
da Costa, Lucas Maciel& Oliveira de Almeida Leite, Heitor& Kassab, Nájla Mohamad& Singh, Anil Kumar. Green Analytical Methods for the Separation of Seven Antihistamines: Application in Separation of Azelastine and Related Impurities in Nasal Solution. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1156007
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156007