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Long-Zhi Decoction Medicated Serum Promotes Angiogenesis in Human Umbilical Vein Endothelial Cells Based on Autophagy
Joint Authors
He, Quan
Chen, Yongbin
Liu, Qihua
Meng, Jiaquan
Zou, Ling
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Ischemic stroke (IS) is a fatal subtype of stroke that lacks effective treatments.
Angiogenesis following IS is an effective response that mediates brain recovery and repair.
Our previous study demonstrated that long-zhi decoction (LZD), a Chinese herbal formula, promoted angiogenesis in rats of IS model.
To further investigate the association between the proangiogenic mechanism of an LZD-medicated serum and cellular autophagy, we evaluated its promotional effect on angiogenesis in human umbilical vein endothelial cells (HUVECs) in vitro.
We used HUVECs subjected to H2O2 to induce injury and observed the effects of the LZD-medicated serum treatment.
Cell-based assays included proliferation, migration, and tube formation.
To assess the extent of autophagy, transmission electron microscopy was used to measure the number of autophagosomes.
Immunofluorescence and Western blotting were performed to evaluate the autophagy-related protein of LC3-II and Beclin-1.
The LZD-medicated serum promoted proliferation, migration, and tube formation in HUVECs.
The LZD-medicated serum also increased the autophagosomes and the autophagic protein expressions of LC3-II and Beclin-1.
The proangiogenic and autophagic activity of LZD provides new cogitations to its clinical application and may lead to potential drug development for treating various vascular diseases, especially in the elderly, in the future.
American Psychological Association (APA)
He, Quan& Liu, Qihua& Chen, Yongbin& Meng, Jiaquan& Zou, Ling. 2018. Long-Zhi Decoction Medicated Serum Promotes Angiogenesis in Human Umbilical Vein Endothelial Cells Based on Autophagy. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156054
Modern Language Association (MLA)
He, Quan…[et al.]. Long-Zhi Decoction Medicated Serum Promotes Angiogenesis in Human Umbilical Vein Endothelial Cells Based on Autophagy. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1156054
American Medical Association (AMA)
He, Quan& Liu, Qihua& Chen, Yongbin& Meng, Jiaquan& Zou, Ling. Long-Zhi Decoction Medicated Serum Promotes Angiogenesis in Human Umbilical Vein Endothelial Cells Based on Autophagy. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156054
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156054