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In Vitro Antidiabetic Effects of Isolated Triterpene Glycoside Fraction from Gymnema sylvestre
Joint Authors
Shenoy, Rashmi S.
Prashanth, K. V. Harish
Manonmani, H. K.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-08
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A triterpene glycoside (TG) fraction isolated and purified from ethanolic extract of Gymnema sylvestre (EEGS) was investigated for blood glucose control benefit using in vitro methods.
The HPLC purified active fraction TG was characterized using FTIR, LC-MS, and NMR.
The purified fraction (TG) exhibited effective inhibition of yeast α-glucosidase, sucrase, maltase, and pancreatic α-amylase with IC50 values 3.16 ± 0.05 μg/mL, 74.07 ± 0.51, 5.69 ± 0.02, and 1.17 ± 0.24 μg/mL, respectively, compared to control.
TG was characterized to be a mixture of triterpene glycosides: gymnemic acids I, IV, and VII and gymnemagenin.
In vitro studies were performed using mouse pancreatic β-cell lines (MIN6).
TG did not exhibit any toxic effects on β-cell viability and showed protection against H2O2 induced ROS generation.
There was up to 1.34-fold increase in glucose stimulated insulin secretion (p<0.05) in a dose-dependent manner relative to standard antidiabetic drug glibenclamide.
Also, there was further one-fold enhancement in the expression of GLUT2 compared to commercial standard DAG (deacylgymnemic acid).
Thus, the present study highlights the effective isolation and therapeutic potential of TG, making it a functional food ingredient and a safe nutraceutical candidate for management of diabetes.
American Psychological Association (APA)
Shenoy, Rashmi S.& Prashanth, K. V. Harish& Manonmani, H. K.. 2018. In Vitro Antidiabetic Effects of Isolated Triterpene Glycoside Fraction from Gymnema sylvestre. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1156099
Modern Language Association (MLA)
Shenoy, Rashmi S.…[et al.]. In Vitro Antidiabetic Effects of Isolated Triterpene Glycoside Fraction from Gymnema sylvestre. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1156099
American Medical Association (AMA)
Shenoy, Rashmi S.& Prashanth, K. V. Harish& Manonmani, H. K.. In Vitro Antidiabetic Effects of Isolated Triterpene Glycoside Fraction from Gymnema sylvestre. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1156099
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156099