Propolin C Inhibited Migration and Invasion via Suppression of EGFR-Mediated Epithelial-to-Mesenchymal Transition in Human Lung Cancer Cells

Abstract EN

Controlling lung cancer cell migration and invasion via epithelial-to-mesenchymal transition (EMT) through the regulation of epidermal growth factor receptor (EGFR) signaling pathway has been demonstrated.

Searching biological active phytochemicals to repress EGFR-regulated EMT might prevent lung cancer progression.

Propolis has been used as folk medicine in many countries and possesses anti-inflammatory, antioxidant, and anticancer activities.

In this study, the antimigration and anti-invasion activities of propolin C, a c-prenylflavanone from Taiwanese propolis, were investigated on EGFR-regulated EMT signaling pathway.

Cell migration and invasion activities were dose-dependently suppressed by noncytotoxic concentration of propolin C.

Downregulations of vimentin and snail as well as upregulation of E-cadherin expressions were through the inhibition of EGFR-mediated phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) and extracellular signal-regulated kinase (ERK) signaling pathway in propolin C-treated cells.

In addition, EGF-induced migration and invasion were suppressed by propolin C-treated A549 lung cancer cells.

No significant differences in E-cadherin expression were observed in EGF-stimulated cells.

Interestingly, EGF-induced expressions of vimentin, snail, and slug were suppressed through the inhibition of PI3K/Akt and ERK signaling pathway in propolin C-treated cells.

Inhibition of cell migration and invasion by propolin C was through the inhibition of EGF/EGFR-mediated signaling pathway, followed by EMT suppression in lung cancer.