Investigation of the Effects and Mechanisms of Dendrobium loddigesii Rolfe Extract on the Treatment of Gout
Joint Authors
Zhang, Kai-hui
Wang, Mei-qi
Wei, Lu-ling
Feng, Cheng-jing
Zhang, Yu-si
Teng, Jian-bei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
Gout is a chronic disease that causes inflammatory arthritis, which is closely related to urate accumulation induced by a disorder of uric acid metabolism and the consequent deposition of monosodium urate crystals.
Dendrobium loddigesii Rolfe is an herbal medicine that has been used in some traditional Chinese medicine formulae in the treatment of gout.
This study aimed to explore and verify the antigout activity of Dendrobium loddigesii extract (DLE) on alleviating the hyperuricaemia of mice and the acute gouty arthritis of rats.
Methods.
An animal model of hyperuricaemia was established using potassium oxonate (PO).
We analysed the expression of uric acid transporter mRNA in the kidney in the hyperuricaemic mice after treatment with DLE.
Simultaneously, a monosodium urate crystal-induced acute gouty arthritis rat model was used to evaluate the effects of DLE, according to the level of ankle swelling, as well as the protein levels of inflammatory receptors and cytokines, as assayed by WB and ELISA.
Results.
DLE alleviated hyperuricaemia in mice and inhibited acute gouty arthritis in rats (P<0.05).
Meanwhile, DLE regulated the levels of uric acid transporters mRNA transcripts, including mouse organic anion transporter 1 (mOAT1), organic anion transporter 3 (mOAT3), urate transporter 1 (mURAT1), and glucose transporter 9 (mGLUT9) in the kidney (P<0.05), suggesting that DLE promoted uric acid metabolism.
Furthermore, DLE significantly suppressed the protein levels of TLRs, MyD88, and NF-κB in the ankle joint’s synovium (P<0.05), and the serum levels of IL-1β, IL-6, and TNF-α were also reduced, which demonstrated the anti-inflammatory effects of DLE.
Conclusion.
DLE alleviates hyperuricaemia by regulating the transcription level of uric acid transporters in the kidney.
It also inhibits acute gouty arthritis by inhibiting the pathway of TLRs/MyD88/NF-κB in the ankle joint’s synovium.
The findings of the present study imply that DLE alleviates gout by promoting uric acid metabolism and inhibiting inflammation related to the TLRs/MyD88/NF-κB pathway.
American Psychological Association (APA)
Zhang, Kai-hui& Wang, Mei-qi& Wei, Lu-ling& Feng, Cheng-jing& Zhang, Yu-si& Teng, Jian-bei. 2020. Investigation of the Effects and Mechanisms of Dendrobium loddigesii Rolfe Extract on the Treatment of Gout. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156124
Modern Language Association (MLA)
Zhang, Kai-hui…[et al.]. Investigation of the Effects and Mechanisms of Dendrobium loddigesii Rolfe Extract on the Treatment of Gout. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156124
American Medical Association (AMA)
Zhang, Kai-hui& Wang, Mei-qi& Wei, Lu-ling& Feng, Cheng-jing& Zhang, Yu-si& Teng, Jian-bei. Investigation of the Effects and Mechanisms of Dendrobium loddigesii Rolfe Extract on the Treatment of Gout. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156124
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156124