Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells
Joint Authors
Alkhuriji, Afrah F.
Khalil, Mahmoud I.
Abdel Rahman, Rana M.
Ghaffar, Hedayat Abdel
Amaly, Noha
El-Kott, Attalla F.
Sultan, Ahmed S.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-28
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Traditional folk therapies indicate that insects have diverse medicinal potentials.
However, the therapeutic application of insect chitosan and its derivatives has not been explored.
To investigate the application of chitosan and its derivatives, the carboxymethyl derivative of chitosan (CM-Ch) was extracted from two dipteran larvae species, Chrysomya albiceps and Sarcophaga aegyptiaca.
The degree of deacetylation (DD) and CM-Ch functional groups were validated using Fourier-transform infrared (FTIR) spectroscopy analysis and proton nuclear magnetic resonance spectroscopy (1H NMR), respectively.
The molecular weight was estimated using MALDI-TOF MS analysis.
The effect of CM-Ch on the morphology and proliferation of human liver HepG2 cancer cells was assessed.
IC50 of CM-Ch induced significant growth-inhibitory effects in HepG2 cells.
CM-Ch treatment altered the morphology of HepG2 in a dose-dependent manner and induced apoptosis in a caspase-dependent manner.
CM-Ch treatment showed no signs of toxicity, and no alterations in liver and kidney biochemical markers were observed in albino rats.
A CM-Ch derivative from commercial crustacean chitosan was used to assess the efficacy of the insect-derived CM-Ch.
The data presented here introduce insect CM-Ch as a promising, inexhaustible, safe derivative of chitosan with antitumor potential in liver cancer.
This is the first report highlighting the anticancer activity of insect CM-Ch in hepatocellular carcinoma cells.
American Psychological Association (APA)
Abdel Rahman, Rana M.& Ghaffar, Hedayat Abdel& Alkhuriji, Afrah F.& Khalil, Mahmoud I.& Amaly, Noha& El-Kott, Attalla F.…[et al.]. 2020. Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1156147
Modern Language Association (MLA)
Abdel Rahman, Rana M.…[et al.]. Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1156147
American Medical Association (AMA)
Abdel Rahman, Rana M.& Ghaffar, Hedayat Abdel& Alkhuriji, Afrah F.& Khalil, Mahmoud I.& Amaly, Noha& El-Kott, Attalla F.…[et al.]. Dipteran Carboxymethyl Chitosan as an Inexhaustible Derivative with a Potential Antiproliferative Activity in Hepatocellular Carcinoma Cells. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1156147
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156147