A Downmodulated MicroRNA Profiling in Patients with Gastric Cancer
Joint Authors
Zhang, Tao
Liu, Chang
Huang, Shi
Ma, Yuanping
Fang, Jiansong
Chen, Yuanneng
Source
Gastroenterology Research and Practice
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
Here, we aim to investigate the microRNA (miR) profiling in human gastric cancer (GC).
Methods.
Tumoral and matched peritumoral gastric specimens were collected from 12 GC patients who underwent routine surgery.
A high-throughput miR sequencing method was applied to detect the aberrantly expressed miRs in a subset of 6 paired samples.
The stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) assay was subsequently performed to confirm the sequencing results in the remaining 6 paired samples.
The profiling results were also validated in vitro in three human GC cell lines (BGC-823, MGC-803, and GTL-16) and a normal gastric epithelial cell line (GES-1).
Results.
The miR sequencing approach detected 5 differentially expressed miRs, hsa-miR-132-3p, hsa-miR-155-5p, hsa-miR-19b-3p, hsa-miR-204-5p, and hsa-miR-30a-3p, which were significantly downmodulated between the tumoral and peritumoral GC tissues.
Most of the results were further confirmed by qRT-PCR, while no change was observed for hsa-miR-30a-3p.
The in vitro finding also agreed with the results of both miR sequencing and qRT-PCR for hsa-miR-204-5p, hsa-miR-155-5p, and hsa-miR-132-3p.
Conclusion.
Together, our findings may serve to identify new molecular alterations as well as to enrich the miR profiling in human GC.
American Psychological Association (APA)
Zhang, Tao& Liu, Chang& Huang, Shi& Ma, Yuanping& Fang, Jiansong& Chen, Yuanneng. 2017. A Downmodulated MicroRNA Profiling in Patients with Gastric Cancer. Gastroenterology Research and Practice،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1156153
Modern Language Association (MLA)
Zhang, Tao…[et al.]. A Downmodulated MicroRNA Profiling in Patients with Gastric Cancer. Gastroenterology Research and Practice No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1156153
American Medical Association (AMA)
Zhang, Tao& Liu, Chang& Huang, Shi& Ma, Yuanping& Fang, Jiansong& Chen, Yuanneng. A Downmodulated MicroRNA Profiling in Patients with Gastric Cancer. Gastroenterology Research and Practice. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1156153
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156153