Electroacupuncture Pretreatment Alleviates LPS-Induced Acute Respiratory Distress Syndrome via Regulating the PPAR GammaNF-Kappa B Signaling Pathway
Joint Authors
Song, Jiangang
Lv, Xin
Shi, Xuan
Feng, Di
Zhou, Huanping
Jin, Xiaohong
Wei, Juan
Zhang, Qingqing
Gu, Yang
Zhang, Pengcheng
Yang, Hao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Electroacupuncture (EA) is reported to possess anti-inflammatory properties and has beneficial effects on acute respiratory distress syndrome (ARDS).
However, the underlying mechanisms of the effects of EA on ARDS remain unclear.
This study aims to investigate the protective effect of EA on LPS-induced ARDS.
In this study, Sprague-Dawley male rats were treated with EA at Hegu (LI4) for 45 minutes before LPS instillation (0.4 mg/kg, 100 ul).
H&E staining, wet-to-dry weight (W/D) ratio, PaO2, and protein content in BALF were employed to determine the function of lung tissues.
Inflammatory cytokines in serum and BALF were detected by enzyme-linked immunoassay assay (ELISA).
The levels of oxidative stress markers were detected to determine the oxidative stress status.
Cell apoptosis was observed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining and western blot.
Here, we found that EA pretreatment effectively alleviated lung pathological damage.
Moreover, EA suppressed the oxidative stress damage by upregulating glutathione and superoxide dismutase and downregulating malondialdehyde.
EA pretreatment also regulated apoptosis-related proteins, such as Bax and Bcl-2.
We found that peroxisome proliferators-activated receptors γ (PPARγ) play a critical role during ARDS, EA up-regulated the expression of PPARγ, which inhibited the activation of nuclear factor-kappa B (NF-κB) and decreased the inflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor-α).
When rats were treated with GW9662, a selective PPARγ antagonist, these effects of EA were reversed.
Our study demonstrated that EA pretreatment had a beneficial effect on LPS-induced ARDS in rats by anti-inflammatory, antioxidative, and antiapoptotic properties which was regulated via PPARγ/NF-κB signaling pathway.
American Psychological Association (APA)
Feng, Di& Zhou, Huanping& Jin, Xiaohong& Wei, Juan& Zhang, Qingqing& Gu, Yang…[et al.]. 2020. Electroacupuncture Pretreatment Alleviates LPS-Induced Acute Respiratory Distress Syndrome via Regulating the PPAR GammaNF-Kappa B Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156208
Modern Language Association (MLA)
Feng, Di…[et al.]. Electroacupuncture Pretreatment Alleviates LPS-Induced Acute Respiratory Distress Syndrome via Regulating the PPAR GammaNF-Kappa B Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156208
American Medical Association (AMA)
Feng, Di& Zhou, Huanping& Jin, Xiaohong& Wei, Juan& Zhang, Qingqing& Gu, Yang…[et al.]. Electroacupuncture Pretreatment Alleviates LPS-Induced Acute Respiratory Distress Syndrome via Regulating the PPAR GammaNF-Kappa B Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156208
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156208