In Vitro Antioxidant Potential and Effect of a Glutathione-Enhancer Dietary Supplement on Selected Rat Liver Cytochrome P450 Enzyme Activity

Joint Authors

Kukuia, Kennedy Kwami Edem
Appiah-Opong, Regina
N’guessan, Benoit Banga
Amponsah, Seth Kwabena
Asiedu-Gyekye, Isaac Julius
Dugbartey, George J.
Awuah, Kwabena D.
Dotse, Eunice
Aning, Abigail

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2018-05-24

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

Background.

There is considerable evidence that many people take dietary supplements including those of herbal origin as an alternative therapy to improve their health.

One such supplement, with an amalgam of constituents, is CellGevity®.

However, the effect of this dietary supplement on drug-metabolizing enzymes is poorly understood, as it has not been studied extensively.

Therefore, we investigated the effect of CellGevity dietary supplement on selected rat liver microsomal cytochrome P450 (CYP) enzymes, the most common drug-metabolizing enzymes.

We also determined the total antioxidant potential of this dietary supplement in vitro.

Methods.

To determine the antioxidant potential of CellGevity dietary supplement, 2,2-diphenyl-2-picryl-hydrazyl (DPPH), total phenolic, and flavonoid assays were used after initial preparation of a solution form of the supplement (low dose, LD; 4 mg/kg and high dose, HD; 8 mg/kg).

Rats received oral administration of these doses of the supplement for 7 days, after which the effect of the supplement on selected liver CYP enzymes was assessed using probe substrates and spectroscopic and high-performance liquid chromatographic methods.

Rats which received daily administration of 80 mg/kg of phenobarbitone and distilled water served as positive and negative controls, respectively.

Results.

The IC50 value of the supplement 0.34 ± 0.07 mg/ml compared to 0.076±0.03 mg/ml of the BHT (positive control).

The total phenolic content of the supplement at a concentration of 2.5 mg/ml was 34.97 g gallic acid equivalent (GAE)/100 g while its total flavonoid content at a concentration of 2.5 mg/ml was 6 g quercetin equivalent (QE)/100 g.

The supplement significantly inhibited rat CYP2B1/2B2 (LDT 92.4%; HDT 100%), CYP3A4 (LDT 81.2%; HDT 71.7%), and CYP2C9 (LDT 21.7%; HDT 28.5%) while it had no significant inhibitory effect on CYPs 1A1/1A2, CYP1A2, and CYP2D6.

Conclusion.

CellGevity dietary supplement possesses moderate antioxidant activity in vitro and has an inhibitory effect on selected rat liver CYP enzymes, suggesting its potential interaction with drugs metabolized by CYP enzymes.

American Psychological Association (APA)

N’guessan, Benoit Banga& Amponsah, Seth Kwabena& Dugbartey, George J.& Awuah, Kwabena D.& Dotse, Eunice& Aning, Abigail…[et al.]. 2018. In Vitro Antioxidant Potential and Effect of a Glutathione-Enhancer Dietary Supplement on Selected Rat Liver Cytochrome P450 Enzyme Activity. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1156219

Modern Language Association (MLA)

N’guessan, Benoit Banga…[et al.]. In Vitro Antioxidant Potential and Effect of a Glutathione-Enhancer Dietary Supplement on Selected Rat Liver Cytochrome P450 Enzyme Activity. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1156219

American Medical Association (AMA)

N’guessan, Benoit Banga& Amponsah, Seth Kwabena& Dugbartey, George J.& Awuah, Kwabena D.& Dotse, Eunice& Aning, Abigail…[et al.]. In Vitro Antioxidant Potential and Effect of a Glutathione-Enhancer Dietary Supplement on Selected Rat Liver Cytochrome P450 Enzyme Activity. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1156219

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1156219