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Using Phytochemicals to Investigate the Activation of Nicotine Detoxification via Upregulation of CYP2A6 in Animal Models Exposed Tobacco Smoke Condensate by Intratracheal Instillation
Joint Authors
Shim, Soon-Mi
Park, Tae-Sik
Lee, Dayeon
Seo, Seung-Beom
Lee, Hyun Jeong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-19
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
This study examined the efficacy of standardized Smilax china L.
root extract (SSCR) containing chlorogenic acid on detoxifying nicotine from tobacco smoke condensate (TSC) in vitro and in vivo.
Chlorogenic acid is an identified bioactive component in SSCR by ultraperformance liquid chromatography/photodiode array/electrospray ionization/mass spectroscopy (UPLC/PDA/ESI/MS).
HepG2 liver cells and A549 lung cells were carried for measuring ROS and antioxidant enzymes.
Sprague-Dawley rats were treated with nicotine by intratracheal instillation (ITI).
Cell viabilities by pretreatments of 5, 12.5, and 25, 50 μg SSCR/mL ranged from 41 to 76% in HepG2 and 65 to 95% in A549.
Pretreatments of SSCR inhibited TSC-mediated production of reactive oxygen species (ROS) by 8 and 10% in HepG2 and A549 cells, respectively.
However, the expression of CAT, SOD1, and AOX1 was downregulated by SSCR in the both cells.
The highest conversion of cotinine was observed at 50 μg/mL of SSCR after 120 min of incubation.
SSCR upregulated CYP2A6 3-fold in A549 cells regardless of TSC cotreatment.
When Sprague-Dawley rats were treated with nicotine by ITI or subjected to SSCR administration for 14 days, the levels of cotinine in urine increased in SSCR treatment only.
The cellular level of antioxidant capacity at 10 or 100 mg/kg body weight/day of SSCR treatment was 1.89 and 1.86 times higher than those of nicotine-control.
Results suggest that the intake of SSCR can detoxify nicotine by elevating nicotine conversion to cotinine and antioxidant capacity.
American Psychological Association (APA)
Lee, Dayeon& Seo, Seung-Beom& Lee, Hyun Jeong& Park, Tae-Sik& Shim, Soon-Mi. 2018. Using Phytochemicals to Investigate the Activation of Nicotine Detoxification via Upregulation of CYP2A6 in Animal Models Exposed Tobacco Smoke Condensate by Intratracheal Instillation. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1156262
Modern Language Association (MLA)
Lee, Dayeon…[et al.]. Using Phytochemicals to Investigate the Activation of Nicotine Detoxification via Upregulation of CYP2A6 in Animal Models Exposed Tobacco Smoke Condensate by Intratracheal Instillation. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1156262
American Medical Association (AMA)
Lee, Dayeon& Seo, Seung-Beom& Lee, Hyun Jeong& Park, Tae-Sik& Shim, Soon-Mi. Using Phytochemicals to Investigate the Activation of Nicotine Detoxification via Upregulation of CYP2A6 in Animal Models Exposed Tobacco Smoke Condensate by Intratracheal Instillation. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1156262
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156262