Manilkara zapota (L.) P. Royen Leaf Water Extract Induces Apoptosis in Human Hepatocellular Carcinoma (HepG2) Cells via ERK12Akt1JNK1 Signaling Pathways
Joint Authors
Tan, Bee Ling
Norhaizan, Mohd Esa
Chan, Lee Chin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-05
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Manilkara zapota (L.) P.
Royen, called sapodilla, or locally known as ciku, belongs to the family Sapotaceae.
We found that Manilkara zapota leaf water extract has cytotoxic effect against human hepatocellular carcinoma (HepG2) cell line in our earlier study.
Therefore, this study aimed to explore the anticancer properties of Manilkara zapota leaf water extract in HepG2 cells.
We also aimed to unravel yet undiscovered mechanisms and identified several expressed genes whose functions in cytotoxicity activity of Manilkara zapota leaf water extract in HepG2 cells have not been well-studied.
The apoptosis and intracellular reactive oxygen species (ROS) activities were analyzed using Annexin V-propidium iodide staining and dichlorodihydrofluorescein diacetate, respectively, by NovoCyte Flow Cytometer.
Bax and Bcl-2 expression were assessed using Enzyme-Linked Immunosorbent Assay.
The associated molecular pathways were evaluated by quantitative real-time PCR.
Overall analyses revealed that Manilkara zapota leaf water extract can increase percentage of early apoptotic cells, induce the formation of ROS, upregulate c-Jun N-terminal kinase 1 (JNK1) and inducible nitric oxide synthase (iNOS), and reduce Akt1 and vascular endothelial growth factor A (VEGFA) transcriptional activities.
Our data suggest that Manilkara zapota leaf water extract can suppress the growth of HepG2 cells via modulation of ERK1/2/Akt1/JNK1 transcriptional expression.
American Psychological Association (APA)
Tan, Bee Ling& Norhaizan, Mohd Esa& Chan, Lee Chin. 2018. Manilkara zapota (L.) P. Royen Leaf Water Extract Induces Apoptosis in Human Hepatocellular Carcinoma (HepG2) Cells via ERK12Akt1JNK1 Signaling Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1156279
Modern Language Association (MLA)
Tan, Bee Ling…[et al.]. Manilkara zapota (L.) P. Royen Leaf Water Extract Induces Apoptosis in Human Hepatocellular Carcinoma (HepG2) Cells via ERK12Akt1JNK1 Signaling Pathways. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-17.
https://search.emarefa.net/detail/BIM-1156279
American Medical Association (AMA)
Tan, Bee Ling& Norhaizan, Mohd Esa& Chan, Lee Chin. Manilkara zapota (L.) P. Royen Leaf Water Extract Induces Apoptosis in Human Hepatocellular Carcinoma (HepG2) Cells via ERK12Akt1JNK1 Signaling Pathways. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-17.
https://search.emarefa.net/detail/BIM-1156279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156279