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Electroacupuncture Mitigates Skeletal Muscular Lipid Metabolism Disorder Related to High-Fat-Diet Induced Insulin Resistance through the AMPKACC Signaling Pathway
Joint Authors
Sun, Jian
Li, Zhixing
Lan, Danchun
Zhang, Haihua
Zhang, Hongtao
Chen, Xiaozhuan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of this work is to investigate the effect of electroacupuncture (EA) on insulin sensitivity in high-fat diet (HFD) induced insulin resistance (IR) rats and to evaluate expression of AMPK/ACC signaling components.
Thirty-two male Sprague-Dawley rats were randomized into control group, HFD group, HFD+Pi (oral gavage of pioglitazone) group, and HFD+EA group.
Acupuncture was subcutaneously applied to Zusanli (ST40) and Sanyinjiao (SP6).
For Zusanli (ST40) and Sanyinjiao (SP6), needles were connected to an electroacupuncture (EA) apparatus.
Fasting plasma glucose was measured by glucose oxidase method.
Plasma fasting insulin (FINS) and adiponectin (ADP) were determined by ELISA.
Triglyceride (TG) and cholesterol (TC) were determined by Gpo-pap.
Proteins of adiponectin receptor 1 (adipoR1), AMP-activated Protein Kinase (AMPK), and acetyl-CoA carboxylase (ACC) were determined by Western blot, respectively.
Compared with the control group, HFD group exhibits increased levels of FPG, FINS, and homeostatic model assessment of insulin resistance (HOMA-IR) and decreased level of ADP and insulin sensitivity index (ISI).
These changes were reversed by both EA and pioglitazone.
Proteins of adipoR1 and AMPK were decreased, while ACC were increased in HFD group compared to control group.
Proteins of these molecules were restored back to normal levels upon EA and pioglitazone.
EA can improve the insulin sensitivity of insulin resistance rats; the positive regulation of the AMPK/ACC pathway in the skeletal muscle may be a possible mechanism of EA in the treatment of IR.
American Psychological Association (APA)
Li, Zhixing& Lan, Danchun& Zhang, Haihua& Zhang, Hongtao& Chen, Xiaozhuan& Sun, Jian. 2018. Electroacupuncture Mitigates Skeletal Muscular Lipid Metabolism Disorder Related to High-Fat-Diet Induced Insulin Resistance through the AMPKACC Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1156318
Modern Language Association (MLA)
Li, Zhixing…[et al.]. Electroacupuncture Mitigates Skeletal Muscular Lipid Metabolism Disorder Related to High-Fat-Diet Induced Insulin Resistance through the AMPKACC Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1156318
American Medical Association (AMA)
Li, Zhixing& Lan, Danchun& Zhang, Haihua& Zhang, Hongtao& Chen, Xiaozhuan& Sun, Jian. Electroacupuncture Mitigates Skeletal Muscular Lipid Metabolism Disorder Related to High-Fat-Diet Induced Insulin Resistance through the AMPKACC Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1156318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156318