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The Value of Caspase-3 after the Application of Annona muricata Leaf Extract in COLO-205 Colorectal Cancer Cell Line
Joint Authors
Syam, Ari Fahrial
Abdullah, Murdani
Meilany, Sofy
Laksono, Bayu
Prabu, Oryza Gryagus
Bekti, Heri Setiyo
Indrawati, Lili
Makmun, Dadang
Source
Gastroenterology Research and Practice
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-09
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Annona muricata, commonly known as soursop, contains annonacin, acetogenin, and polyphenol which are known to have chemopreventive effects on cancer.
In this study, we tend to evaluate the apoptosis-inducing effect of soursop (Annona muricata) leaf extract on the colorectal cancer cell line COLO-205 through the activities of caspase-3 which is a marker of cell apoptosis.
Cell cultures were incubated with soursop leaf with a concentration of 10 μg/ml and then compared with those of the incubated positive control leucovorin 10 μg/ml and placebo as a negative control.
The apoptotic activity of caspase-3 was measured with ELISA.
After the administration of each treatment in the colorectal cancer cell line COLO-205, the expression of caspase-3 activity was 1422 ng/ml after incubation with the extract of Annona muricata leaves, 1373 ng/ml after the administration of leucovorin, and 1297 ng/ml in the one with placebo.
Annona muricata leaf extract elevated caspase-3 by 1.09 times compared to that of the pure cell line.
Annona muricata leaf extract had a higher value of caspase-3 activity than leucovorin and placebo in the COLO-205 colorectal cancer cell line.
These results may suggest that Annona muricata leaf extract had anticancer properties by enhancing caspase-3 activity which is a proapoptotic marker.
American Psychological Association (APA)
Abdullah, Murdani& Syam, Ari Fahrial& Meilany, Sofy& Laksono, Bayu& Prabu, Oryza Gryagus& Bekti, Heri Setiyo…[et al.]. 2017. The Value of Caspase-3 after the Application of Annona muricata Leaf Extract in COLO-205 Colorectal Cancer Cell Line. Gastroenterology Research and Practice،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1156381
Modern Language Association (MLA)
Abdullah, Murdani…[et al.]. The Value of Caspase-3 after the Application of Annona muricata Leaf Extract in COLO-205 Colorectal Cancer Cell Line. Gastroenterology Research and Practice No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1156381
American Medical Association (AMA)
Abdullah, Murdani& Syam, Ari Fahrial& Meilany, Sofy& Laksono, Bayu& Prabu, Oryza Gryagus& Bekti, Heri Setiyo…[et al.]. The Value of Caspase-3 after the Application of Annona muricata Leaf Extract in COLO-205 Colorectal Cancer Cell Line. Gastroenterology Research and Practice. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1156381
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156381