Expression of miRNAs in Serum Exosomes versus Hippocampus in Methamphetamine-Induced Rats and Intervention of Rhynchophylline
Joint Authors
Li, Han-cheng
Lin, Ying-bo
Li, Chan
Luo, Chao-hua
Zhou, Yu-ting
Ou, Jing-ying
Li, Jing
Mo, Zhi-xian
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
To compare the expressions of miRNAs (microRNAs) in serum exosomes and in hippocampus and to provide insights into the miRNA-mediated relationship between peripheral and central nervous systems in the presence of methamphetamine.
Methods.
Published results on conditioned place preference (CPP) in rats conditioned by methamphetamine were replicated.
The expressions of miRNAs in serum exosomes and hippocampus were determined by gene-chip sequencing.
We then predicted the potential target genes of selected, differentially expressed (DE) miRNAs and then carried out functional analysis of these target genes.
We also verified our results by RT-qPCR.
Results.
Methamphetamine reward could greatly increase the activity time and distance in the intrinsically nonpreferred side of the behavioral apparatus compared with control rats (P<0.01).
Rhynchophylline treatment significantly counteracted these changes (P<0.01).
Methamphetamine-induced CPP upregulated 23 miRNAs (log2 fold change [FC] > 1, P<0.01) in serum exosomes, whereas rhynchophylline treatment could downregulate these miRNAs (log2 FC < −1, P<0.01).
Analysis of hippocampal miRNAs profiles found 22 DE miRNAs (log2 FC > 1 or <−1, P<0.01).
When methamphetamine induced CPP, 11 of those miRNAs were upregulated, whereas rhynchophylline treatment could downregulate these miRNAs.
The other 11 miRNAs behaved in the opposite way.
We selected six DE miRNAs from each of serum exosomes and hippocampus for target gene prediction and functional analysis.
We found that, in both, the DE miRNAs and their target genes may be related to neuronal information transmission and synaptic transmission.
Conclusions.
Rhynchophylline blocked the alteration of behavior and the expression of some DE miRNAs induced by methamphetamine.
The biological functions of these DE miRNAs target genes are correlated between serum exosomes and hippocampus.
As to these biological processes and pathways which are involved in the development of addiction at multiple stages, we speculate that these DE miRNAs in serum exosomes and hippocampus are closely related to methamphetamine addiction.
American Psychological Association (APA)
Li, Han-cheng& Lin, Ying-bo& Li, Chan& Luo, Chao-hua& Zhou, Yu-ting& Ou, Jing-ying…[et al.]. 2018. Expression of miRNAs in Serum Exosomes versus Hippocampus in Methamphetamine-Induced Rats and Intervention of Rhynchophylline. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156383
Modern Language Association (MLA)
Li, Han-cheng…[et al.]. Expression of miRNAs in Serum Exosomes versus Hippocampus in Methamphetamine-Induced Rats and Intervention of Rhynchophylline. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1156383
American Medical Association (AMA)
Li, Han-cheng& Lin, Ying-bo& Li, Chan& Luo, Chao-hua& Zhou, Yu-ting& Ou, Jing-ying…[et al.]. Expression of miRNAs in Serum Exosomes versus Hippocampus in Methamphetamine-Induced Rats and Intervention of Rhynchophylline. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1156383
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156383