Neuroprotection of Tropical Fruit Juice Mixture via the Reduction of iNOS Expression and CRH Level in β-Amyloid-Induced Rats Model of Alzheimer’s Disease
Joint Authors
Shahar, Suzana
Rajab, Nor Fadilah
Ooi, Theng Choon
Ahmad Munawar, Munirah
Mohd Rosli, Nur Hasnieza
Abdul Malek, Siti Nur Aqilah
Rosli, Hanisah
Ibrahim, Farah Wahida
Azmi, Norazrina
Haron, Hasnah
Sharif, Razinah
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study aimed to determine the effects of tropical fruit juice mixture (pomegranate, white guava, and Roselle) on biochemical, behavioral, and histopathological changes of β-amyloid- (Aβ-) induced rats.
Formulation 8 (F8) of tropical fruit juice mixture was chosen for this present study due to its high phenolic content and antioxidant capacity.
Forty Wistar male rats were divided into five groups: dPBS (sham-operated control), dAβ (Aβ control), JPBS (F8 and PBS), JAβ (F8 and Aβ), and IBFAβ (ibuprofen and Aβ).
F8 (5 ml/kg BW), and ibuprofen (10 ml/kg BW) was given orally daily for four weeks before the intracerebroventricular infusion of Aβ for two weeks.
Histological analysis and neuronal count of hippocampus tissue in the Cornu Ammonis (CA1) region showed that supplementation with F8 was able to prevent Aβ-induced tissue damage and neuronal shrinkage.
However, no significant difference in locomotor activity and novel object recognition (NOR) percentage was detected among different groups at day 7 and day 14 following Aβ infusion.
Only effect of time differences (main effect of day) was observed at day 7 as compared to day 14, where reduction in locomotor activity and NOR percentage was observed in all groups, with F (1, 7) = 6.940, p<0.05 and F (1, 7) = 7.152, p<0.05, respectively.
Besides, the MDA level of the JAβ group was significantly lower (p<0.01) than that of the dPBS group.
However, no significant changes in SOD activity were detected among different groups.
Significant reduction in plasma CRH level (p<0.05) and iNOS expression (p<0.01) in the brain was detected in the JAβ group as compared to the dAβ group.
Hence, our current findings suggest that the tropical fruit juice mixture (F8) has the potential to protect the rats from Aβ-induced neurotoxicity in brain hippocampus tissue possibly via its antioxidant properties and the suppression of iNOS expression and CRH production.
American Psychological Association (APA)
Ooi, Theng Choon& Ahmad Munawar, Munirah& Mohd Rosli, Nur Hasnieza& Abdul Malek, Siti Nur Aqilah& Rosli, Hanisah& Ibrahim, Farah Wahida…[et al.]. 2020. Neuroprotection of Tropical Fruit Juice Mixture via the Reduction of iNOS Expression and CRH Level in β-Amyloid-Induced Rats Model of Alzheimer’s Disease. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1156426
Modern Language Association (MLA)
Ooi, Theng Choon…[et al.]. Neuroprotection of Tropical Fruit Juice Mixture via the Reduction of iNOS Expression and CRH Level in β-Amyloid-Induced Rats Model of Alzheimer’s Disease. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1156426
American Medical Association (AMA)
Ooi, Theng Choon& Ahmad Munawar, Munirah& Mohd Rosli, Nur Hasnieza& Abdul Malek, Siti Nur Aqilah& Rosli, Hanisah& Ibrahim, Farah Wahida…[et al.]. Neuroprotection of Tropical Fruit Juice Mixture via the Reduction of iNOS Expression and CRH Level in β-Amyloid-Induced Rats Model of Alzheimer’s Disease. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1156426
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156426