Effects of Huanglian Jiedu Decoration in Rat Gingivitis
Joint Authors
Zhao, Haiyu
Bian, Baolin
Geng, Ya
Wang, Hongjie
Zhang, Yi
Li, Defeng
Yang, Hongjun
Zhang, Fangbo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-16
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Gingivitis is an inflammatory disease that affects gingival tissues through a microbe-immune interaction.
Huanglian Jiedu decoction (HLJD) is used traditionally for clearing and detoxifying in China, which had been reported to possess many pharmacological effects.
Rat gingival inflammation model was established by lipopolysaccharide (LPS) injection for 3 consecutive days, and HLJD was given by gavage before LPS injection.
After 3 days rats were sacrificed and tissue samples were evaluated.
Serum cytokine levels such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunoabsorbent assay (ELISA).
Oxidative stress related molecules such as total antioxidant capacity (T-AOC), malondialdehyde (MDA), and reactive oxygen species (ROS) were determined.
Expression of AMP-activated protein kinase (AMPK) and extracellular signal-regulated kinases 1/2 (ERK1/2) signaling pathway were inspected by western blotting.
Histological changes of gingival tissues were tested with hematoxylin-eosin (HE) staining.
HLJD significantly decreased serum levels of IL-6 and TNF-α, suppressed generation of MDA and ROS, and enhanced T-AOC creation.
Moreover, HLJD inhibited expressions of AMPK and ERK1/2.
The inflammation severity of gingival tissue by HE staining was severe in model group but relieved in HLJD group obviously.
HLJD exhibited protective effects against gingival damage through suppressing inflammation reaction and elevating antioxidation power.
American Psychological Association (APA)
Zhang, Fangbo& Geng, Ya& Zhao, Haiyu& Wang, Hongjie& Zhang, Yi& Li, Defeng…[et al.]. 2018. Effects of Huanglian Jiedu Decoration in Rat Gingivitis. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1156475
Modern Language Association (MLA)
Zhang, Fangbo…[et al.]. Effects of Huanglian Jiedu Decoration in Rat Gingivitis. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1156475
American Medical Association (AMA)
Zhang, Fangbo& Geng, Ya& Zhao, Haiyu& Wang, Hongjie& Zhang, Yi& Li, Defeng…[et al.]. Effects of Huanglian Jiedu Decoration in Rat Gingivitis. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1156475
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156475