The Mechanisms of the Herbal Components of CRSAS on HK-2 Cells in a HypoxiaReoxygenation Model Based on Network Pharmacology
Joint Authors
Ye, Naijing
Xie, Dengpiao
Yang, Bing
Li, Mingquan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Acute kidney injury is a global problem, which brings a great burden to the society and family.
The component of rhubarb, Salvia miltiorrhiza, Astragalus membranaceus, and safflower (CRSAS) has been proved as an useful agent to treat acute kidney injury (AKI) patients in China.
Objective.
To assess the effect of CRSAS on human renal tubular epithelial cells (HK-2) after the hypoxia/reoxygenation (H/R) and investigate the potential mechanisms.
Methods.
Network pharmacology was used to predict the potential pathways shared by CRSAS and AKI.
Cell counting kit-8 (CCK-8) was used to assess the HK-2 vitality.
Apoptosis of HK-2 cells was detected by carboxyfluorescein succinimidyl ester/propidium iodide (CFSF/PI) staining.
Expression of GRP78, CHOP, caspase-3, and Bax was detected by western blot and quantitative real-time RT-PCR.
Result.
CRSAS and AKI shared the endoplasmic reticulum stress (ERS) pathway based on network pharmacology analysis.
CRSAS increases the vitality of HK-2 cells and reduces the apoptosis of HK-2 cells induced by H/R injury.
The expression of GRP78 and CHOP in CRSAS groups was lower than that of control groups.
Conclusions.
H/R can induce HK-2 cell apoptosis and ERS.
CRSAS can reduce HK-2 cell apoptosis by inhibiting the ERS.
Therefore, CRSAS might be able to treat kidney disease due to I/R injury.
Animal experiment should be done to further prove our finding.
American Psychological Association (APA)
Ye, Naijing& Xie, Dengpiao& Yang, Bing& Li, Mingquan. 2020. The Mechanisms of the Herbal Components of CRSAS on HK-2 Cells in a HypoxiaReoxygenation Model Based on Network Pharmacology. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156528
Modern Language Association (MLA)
Ye, Naijing…[et al.]. The Mechanisms of the Herbal Components of CRSAS on HK-2 Cells in a HypoxiaReoxygenation Model Based on Network Pharmacology. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1156528
American Medical Association (AMA)
Ye, Naijing& Xie, Dengpiao& Yang, Bing& Li, Mingquan. The Mechanisms of the Herbal Components of CRSAS on HK-2 Cells in a HypoxiaReoxygenation Model Based on Network Pharmacology. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1156528
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156528