Bu Shen Yang Xue Prescription Has Treating Effect on Endometrial Cancer through FSHPI3KAKTGankyrinHIF-αcyclinD 1 Pathway in Ishikawa Cells
Joint Authors
Chen, Yue-qun
Fei, Hua-li
Zhu, Hong-li
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
The formulation of Bu Shen Yang Xue (BSYX) has been clinically used in treating gynecologic disease in China, especially for the development of the endometrium.
Endometrial carcinoma is the most common malignant tumor of the female genital tract in developed countries.
And few studies have been reported on the antitumor activity of BSYX.
Therefore, this study aimed to investigate the effect of BSYX on endometrial cancer and make an initial discussion of the underlining mechanisms in Ishikawa cells.
Methods and Results.
Firstly, 60 SPF female nude mice were randomly divided into control group, model group, BSYX group, and positive group.
The models of subcutaneous tumor xenograft of nude mice were established by injection of human endometrial carcinoma cell line Ishikawa tumor cell suspension.
Compared with model group, BSYX reduced effectively tumor volume and changed pathological feature in mice tumor issue.
Meanwhile, proteins from tumor issues were detected by western blot analysis.
The protein levels of follicle-stimulating hormone receptor (FSHR), p-Akt/Akt, Gankyrin, and cyclinD1 in the model group were higher than those in control group but the expression in BSYX group was lower than that in the model group.
The hypoxia inducible factor alpha (HIF-α) protein level in the model group was lower than those in control group and upregulated in BSYX group.
In addition, Ishikawa cells were cultured and then exposed to follicle-stimulating hormone (FSH), LY294002, a highly selective PI3K inhibitor and serum containing BSYX, respectively.
LY294002 and BSYX markedly decreased the cancer cell viability and migration ability and increased the apoptosis rate.
FSH promoted the cancer cell ability and migration ability.
LY294002 and BSYX evidently downregulated the proteins levels of FSHR, p-Akt/Akt, Gankyrin, and cyclinD1 and upregulated the expression of HIF-α protein, and FSH was on the opposite.
Conclusions.
Taken together, our results showed that the formulation of BSYX had antitumor effect on endometrial cancer in vivo and in vitro and was related with FSH/PI3K/AKT/Gankyrin/HIF-α/cyclinD1 transduction pathway.
American Psychological Association (APA)
Chen, Yue-qun& Fei, Hua-li& Zhu, Hong-li. 2018. Bu Shen Yang Xue Prescription Has Treating Effect on Endometrial Cancer through FSHPI3KAKTGankyrinHIF-αcyclinD 1 Pathway in Ishikawa Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156540
Modern Language Association (MLA)
Chen, Yue-qun…[et al.]. Bu Shen Yang Xue Prescription Has Treating Effect on Endometrial Cancer through FSHPI3KAKTGankyrinHIF-αcyclinD 1 Pathway in Ishikawa Cells. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1156540
American Medical Association (AMA)
Chen, Yue-qun& Fei, Hua-li& Zhu, Hong-li. Bu Shen Yang Xue Prescription Has Treating Effect on Endometrial Cancer through FSHPI3KAKTGankyrinHIF-αcyclinD 1 Pathway in Ishikawa Cells. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1156540
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156540